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Mouse Monoclonal to TNF-alpha

MAb11 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-688-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-688-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-688-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-688-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-688-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-688-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-688-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-688-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.

Specificity

The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).

Regulatory Status

Immunogen

Recombinant human TNF-alpha

Species Reactivity:

  • Human
  • Non-Human Primates
  • Porcine

Negative Species:

Applications:

  • Flow Cytometry
    Application note:intracellular staining
  • Immunohistochemistry (frozen sections)
    Application note:paraformaldehyde-fixed, saponin-treated frozen tissue sections
  • Immunocytochemistry
  • ELISA
    Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1
  • Functional Application
    neutralization
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Wahlstr÷m J, Katchar K, Wigzell H, Olerup O, Eklund A, Grunewald J: Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis. Am J Respir Crit Care Med. 2001 Jan;163(1):115-21. [Abstract] [Full Text]
  • *Yan SR, Qing G, Byers DM, Stadnyk AW, Al-Hertani W, Bortolussi R: Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide. Infect Immun. 2004 Mar;72(3):1223-9. [Abstract] [Full Text]
  • *Visser J, Graffelman W, Blauw B, Haspels I, Lentjes E, de Kloet ER, Nagelkerken L: LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone. J Neuroimmunol. 2001 Oct 1;119(2):343-9. [Abstract]
  • *Cesaro-Tadic S, Dernick G, Juncker D, Buurman G, Kropshofer H, Michel B, Fattinger C, Delamarche E: High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems. Lab Chip. 2004 Dec;4(6):563-9. [Abstract]
  • *Attarbaschi T, Willheim M, Ramharter M, Hofmann A, Wahl K, Winkler H, Graninger W, Winkler S: T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis). Eur Cytokine Netw. 2003 Jan-Mar;14(1):34-9. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Mab11 APC


    Fig. 1. Intracellular staining of activated human peripheral blood with anti-TNF alpha (MAb11) APC.



    MAb11 PE


    Fig. 2. Intracellular staining of activated human peripheral blood with anti-TNF alpha (MAb11) PE.


    1F-688


    Fig. 3. Intracellular staining of CD3-CD28-stimulated buffy coat cells with anti-TNF alpha (MAb11) FITC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO┤s term and conditions which are available at www.exbio.cz.
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