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Mouse Monoclonal to HLA-DR

L243 (IgG2a)

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Background

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system. It is expressed on antigen-presenting cells (dendritic cells, B lymphocytes, monocytes, macrophages).

Specificity

The mouse monoclonal antibody L243 recognizes specifically HLA-DR molecules, both peptide-loaded and empty.

Regulatory Status

Immunogen

Human B lymphocytes

Species Reactivity:

  • Human
  • Non-Human Primates
  • Canine (Dog)

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • Functional Application
    blocking
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Coral S, Pucillo C, Leonardi A, Fonsatti E, Altomonte M, Maio M: Triggering of HLA-DR antigens differentially modulates tumor necrosis factor alpha release by B cells at distinct stage of maturation. Cell Growth Differ. 1997 May;8(5):581-8. [Abstract] [Full Text]
  • *Cantin R, Martin G, Tremblay MJ: A novel virus capture assay reveals a differential acquisition of host HLA-DR by clinical isolates of human immunodeficiency virus type 1 expanded in primary human cells depending on the nature of producing cells and the donor source. J Gen Virol. 2001 Dec;82(Pt 12):2979-87. [Abstract] [Full Text]
  • *Kalka-Moll WM, Tzianabos AO, Bryant PW, Niemeyer M, Ploegh HL, Kasper DL: Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions. J Immunol. 2002 Dec 1;169(11):6149-53. [Abstract]
  • *Muczynski KA, Ekle DM, Coder DM, Anderson SK: Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression. J Am Soc Nephrol. 2003 May;14(5):1336-48. [Abstract] [Full Text]
  • *Bouillon M, El Fakhry Y, Girouard J, Khalil H, Thibodeau J, Mourad W: Lipid raft-dependent and -independent signaling through HLA-DR molecules. J Biol Chem. 2003 Feb 28;278(9):7099-107. [Abstract] [Full Text]
  • *Swiatek-de Lange M, Rist W, Stahl HF, Weith A, Lenter MC: Comment on "MHC class II expression identifies functionally distinct human regulatory T cells". J Immunol. 2008 Mar 15;180(6):3625; author reply 3626. [Abstract] [Full Text]
  • *De Gassart A, Camosseto V, Thibodeau J, Ceppi M, Catalan N, Pierre P, Gatti E: MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation. Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3491-6. [Abstract] [Full Text]
  • *Ivanov A, Beers SA, Walshe CA, Honeychurch J, Alduaij W, Cox KL, Potter KN, Murray S, Chan CH, Klymenko T, Erenpreisa J, Glennie MJ, Illidge TM, Cragg MS: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells. J Clin Invest. 2009 Aug;119(8):2143-59. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    L243 APC-Cy7


    Fig. 1. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) APC-CyTM7.


    L243 PerCP-Cy5-5


    Fig. 2. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) PerCP-CyTM5.5.


    L243 PO


    Fig. 3. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) Pacific OrangeTM.


    L243 PE-Cy7


    Fig. 4. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) PE-CyTM7.


    L243 PE-Cy5


    Fig. 5. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) PE-CyTM5.



    L243 purified


    Fig. 6. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) purified / GAM-APC.


    L243 PE-DyLight 594


    Fig. 7. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) PE-DyLight® 594.


    L243 PE


    Fig. 8. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) PE.

    L243 APC


    Fig. 9. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) APC.


    L243 PB


    Fig. 10. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) Pacific BlueTM.


    L243 FITC


    Fig. 11. Surface staining of HLA-DR in human peripheral blood with anti-HLA-DR (L243) FITC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
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