categories: MHC (Major Histocompatibility Complex) and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to HLA-DR

L243 (IgG2a)

Product No.	Form	Quantity	Price
11-690-C025	purified	0.025 mg	choose region
11-690-C100	purified	0.1 mg	choose region
PB-690-T025	Pacific Blue™	25 tests	choose region
PB-690-T100	Pacific Blue™	100 tests	choose region
1F-690-T025	FITC	25 tests	choose region
1F-690-T100	FITC	100 tests	choose region
1P-690-T025	PE	25 tests	choose region
1P-690-T100	PE	100 tests	choose region
1A-690-T025	APC	25 tests	choose region
1A-690-T100	APC	100 tests	choose region
T9-690-T025	PerCP-Cy™5.5	25 tests	choose region
T9-690-T100	PerCP-Cy™5.5	100 tests	choose region

- In stock! Ready for immediate despatch

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Background

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system. It is expressed on antigen-presenting cells (dendritic cells, B lymphocytes, monocytes, macrophages).

Specificity

The mouse monoclonal antibody L243 recognizes specifically HLA-DR molecules, both peptide-loaded and empty.

Immunogen

Human B lymphocytes

Species Reactivity: Human Non-Human Primates Canine (Dog) Negative Species:	Applications: Flow Cytometry Immunoprecipitation Western Blotting Immunohistochemistry (paraffin sections) Immunohistochemistry (frozen sections) Immunocytochemistry Functional Application blocking Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Coral S, Pucillo C, Leonardi A, Fonsatti E, Altomonte M, Maio M: Triggering of HLA-DR antigens differentially modulates tumor necrosis factor alpha release by B cells at distinct stage of maturation. Cell Growth Differ. 1997 May;8(5):581-8. [Abstract] [Full Text] Cantin R, Martin G, Tremblay MJ: A novel virus capture assay reveals a differential acquisition of host HLA-DR by clinical isolates of human immunodeficiency virus type 1 expanded in primary human cells depending on the nature of producing cells and the donor source. J Gen Virol. 2001 Dec;82(Pt 12):2979-87. [Abstract] [Full Text] Muczynski KA, Ekle DM, Coder DM, Anderson SK: Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression. J Am Soc Nephrol. 2003 May;14(5):1336-48. [Abstract] [Full Text] Bouillon M, El Fakhry Y, Girouard J, Khalil H, Thibodeau J, Mourad W: Lipid raft-dependent and -independent signaling through HLA-DR molecules. J Biol Chem. 2003 Feb 28;278(9):7099-107. [Abstract] [Full Text] Swiatek-de Lange M, Rist W, Stahl HF, Weith A, Lenter MC: Comment on "MHC class II expression identifies functionally distinct human regulatory T cells". J Immunol. 2008 Mar 15;180(6):3625; author reply 3626. [Abstract] [Full Text] De Gassart A, Camosseto V, Thibodeau J, Ceppi M, Catalan N, Pierre P, Gatti E: MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation. Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3491-6. [Abstract] [Full Text] *Ivanov A, Beers SA, Walshe CA, Honeychurch J, Alduaij W, Cox KL, Potter KN, Murray S, Chan CH, Klymenko T, Erenpreisa J, Glennie MJ, Illidge TM, Cragg MS: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells. J Clin Invest. 2009 Aug;119(8):2143-59. [Abstract] [Full Text]

Species Reactivity:

Human
Non-Human Primates
Canine (Dog)

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Western Blotting
Immunohistochemistry (paraffin sections)
Immunohistochemistry (frozen sections)
Immunocytochemistry
Functional Application
blocking

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Coral S, Pucillo C, Leonardi A, Fonsatti E, Altomonte M, Maio M: Triggering of HLA-DR antigens differentially modulates tumor necrosis factor alpha release by B cells at distinct stage of maturation. Cell Growth Differ. 1997 May;8(5):581-8. [Abstract] [Full Text]

*Cantin R, Martin G, Tremblay MJ: A novel virus capture assay reveals a differential acquisition of host HLA-DR by clinical isolates of human immunodeficiency virus type 1 expanded in primary human cells depending on the nature of producing cells and the donor source. J Gen Virol. 2001 Dec;82(Pt 12):2979-87. [Abstract] [Full Text]

*Muczynski KA, Ekle DM, Coder DM, Anderson SK: Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression. J Am Soc Nephrol. 2003 May;14(5):1336-48. [Abstract] [Full Text]

*Bouillon M, El Fakhry Y, Girouard J, Khalil H, Thibodeau J, Mourad W: Lipid raft-dependent and -independent signaling through HLA-DR molecules. J Biol Chem. 2003 Feb 28;278(9):7099-107. [Abstract] [Full Text]

*Swiatek-de Lange M, Rist W, Stahl HF, Weith A, Lenter MC: Comment on "MHC class II expression identifies functionally distinct human regulatory T cells". J Immunol. 2008 Mar 15;180(6):3625; author reply 3626. [Abstract] [Full Text]

*De Gassart A, Camosseto V, Thibodeau J, Ceppi M, Catalan N, Pierre P, Gatti E: MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation. Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3491-6. [Abstract] [Full Text]

*Ivanov A, Beers SA, Walshe CA, Honeychurch J, Alduaij W, Cox KL, Potter KN, Murray S, Chan CH, Klymenko T, Erenpreisa J, Glennie MJ, Illidge TM, Cragg MS: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells. J Clin Invest. 2009 Aug;119(8):2143-59. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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