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Mouse Monoclonal to Nitrotyrosine

EM-30 (IgG1)

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  11-693-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.

Specificity

The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.

Regulatory Status

Immunogen

NO2-Tyr-CH2-Thyroglobulin

Species Reactivity:

  • Broad

Negative Species:

Applications:

  • Western Blotting
    Positive control:NO2-BSA, NO2-HSA
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
    Positive tissue:colon cancer epithelium, prostate hyperplasia
  • Immunohistochemistry (frozen sections)
    Recommended dilution:10 μg/ml
    Positive tissue:colon cancer epithelium, prostate hyperplasia
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

  • Unpublished.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    EM-30 IHC
    Fig. 1.


    Fig. 1. Immunohistochemistry staining (frozen sections) of human tumoral colon epithelium with anti-nitrotyrosine antibody (EM-30).

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