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Mouse Monoclonal to CD45

HI30 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-684-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-684-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PB-684-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
  PB-684-T100 Pacific Blue™ 100 tests yes choose region PDF datasheetHTML datasheet buy
  PO-684-T025 Pacific Orange™ 25 tests yes choose region PDF datasheetHTML datasheet buy
  PO-684-T100 Pacific Orange™ 100 tests yes choose region PDF datasheetHTML datasheet buy
  T9-684-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T9-684-T100 PerCP-Cy™5.5 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

Specificity

The mouse monoclonal antibody HI30 recognizes all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
HLDA IV; WS Code N816

Regulatory Status

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • Functional Application
    blocking CD45 functions
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Yamada T, Zhu D, Saxon A, Zhang K: CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase. J Biol Chem. 2002 Aug 9;277(32):28830-5. [Abstract] [Full Text]
  • *Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW Jr, Arthur LO, Ott DE, Lifson JD: Differential incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and major histocompatibility complex class I and II molecules into human immunodeficiency virus type 1 virions and microvesicles: implications for viral pathogenesis and immune regulation. J Virol. 2001 Jul;75(13):6173-82. [Abstract] [Full Text]
  • *Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function. Eur Heart J. 2005 Jun;26(12):1196-204. [Abstract] [Full Text]
  • *Song L, Ara T, Wu HW, Woo CW, Reynolds CP, Seeger RC, DeClerck YA, Thiele CJ, Sposto R, Metelitsa LS: Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma. J Clin Invest. 2007 Sep;117(9):2702-12. [Abstract] [Full Text]
  • *Cubillos-Ruiz JR, Martinez D, Scarlett UK, Rutkowski MR, Nesbeth YC, Camposeco-Jacobs AL, Conejo-Garcia JR: CD277 is a negative co-stimulatory molecule universally expressed by ovarian cancer microenvironmental cells. Oncotarget. 2010 Sep;1(5):329-38. [Abstract] [Full Text]
  • *Tembhare PR, Subramanian PG, Sehgal K, Yajamanam B, Kumar A, Gadge V, Inamdar N, Gujral S: Immunophenotypic profile of plasma cell leukemia: a retrospective study in a reference cancer center in India and review of literature. Indian J Pathol Microbiol. 2011 Apr-Jun;54(2):294-8. [Abstract] [Full Text]
  • And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    HI30 Pacific Blue FC


    Fig. 1. Surface staining of human peripheral blood cells with anti-human CD45 (HI30) Pacific BlueTM.


    HI30 PO


    Fig. 2. Surface staining of human peripheral blood cells with anti-human CD45 (HI30) Pacific OrangeTM.


    HI30 purified


    Fig. 3. Surface staining of human peripheral blood cells with anti-human CD45 (HI30) purified, GAM-APC.


    HI30 PC5-5


    Fig. 4. Surface staining of human peripheral blood cells with anti-human CD45 (HI30) PerCP-CyTM5.5.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
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    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
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