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Mouse Monoclonal to CD271 / NGFR

NGFR5 (IgG1)

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  11-642-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1B-642-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1P-642-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
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  1A-642-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
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  T9-642-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T9-642-T100 PerCP-Cy™5.5 100 tests yes choose region PDF datasheetHTML datasheet buy
  T7-642-T025 PE-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.

Specificity

The mouse monoclonal antibody NGFR5 (originally C34C) recognizes CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily. The epitope is localized within ammino acids 1 - 160.

Regulatory Status

Immunogen

Purified CD271 protein isolated from human melanoma cell line A875

Species Reactivity:

  • Human
  • Non-Human Primates
  • Feline (Cat)
  • Rabbit
  • Ferret

Negative Species:

  • Mouse
  • Rat

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:1-2 μg/ml
    Positive tissue: melanoma
    Application note: heat-mediated antigen retrieval
  • Immunocytochemistry
  • Mass Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Thompson SJ, Schatteman GC, Gown AM, Bothwell M: A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue. Am J Clin Pathol. 1989 Oct;92(4):415-23. [Abstract]
  • *Marano N, Dietzschold B, Earley JJ Jr, Schatteman G, Thompson S, Grob P, Ross AH, Bothwell M, Atkinson BF, Koprowski H: Purification and amino terminal sequencing of human melanoma nerve growth factor receptor. J Neurochem. 1987 Jan;48(1):225-32. [Abstract]
  • *Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar;8(3):860-73. [Abstract] [Full Text]
  • *Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar;8(3):860-73. [Abstract] [Full Text]
  • *Alpers CE, Hudkins KL, Ferguson M, Johnson RJ, Schatteman GC, Bothwell M: Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys. Lab Invest. 1993 Dec;69(6):703-13. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    NGFR5 MC
    Fig. 1

    Fig. 1. Surface staining (mass cytometry) of brain tumor cells with anti-CD271 (NGFR5) 143Nd. Gated on singlets.


    NGFR5 APC

    Fig. 2. Surface staining (flow cytometry) of REH cells with anti-CD271 (NGFR5) APC.


    NGFR5 PE-Cy7

    Fig. 3. Surface staining (flow cytometry) of REH cells with anti-CD271 (NGFR5) PE-CyTM5.

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