categories: Transcription Regulation & Nuclear Proteins (Human), Transcription Regulation & Nuclear Proteins (Rodent)

Mouse Monoclonal to Notch 1

mN1A (IgG1)

Product No.	Form	Quantity	Price
11-651-C025	purified	0.025 mg	choose region
11-651-C100	purified	0.1 mg	choose region
1P-651-C025	PE	0.025 mg	choose region
1P-651-C100	PE	0.1 mg	choose region
1A-651-C025	APC	0.025 mg	choose region
1A-651-C100	APC	0.1 mg	choose region

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Background

Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged1 (CD339), and Jagged2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.

Entrez Gene: 9q34.3

Specificity

The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

Immunogen

GST fusion protein containing cdc10-NCR region of mouse Notch1

Species Reactivity: Human Mouse Negative Species: Rat	Applications: Flow Cytometry Immunoprecipitation Western Blotting Immunohistochemistry (frozen sections) Immunocytochemistry Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Huppert SS, Le A, Schroeter EH, Mumm JS, Saxena MT, Milner LA, Kopan R: Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1. Nature. 2000 Jun 22;405(6789):966-70. [Abstract] Tagami S, Okochi M, Yanagida K, Ikuta A, Fukumori A, Matsumoto N, Ishizuka-Katsura Y, Nakayama T, Itoh N, Jiang J, Nishitomi K, Kamino K, Morihara T, Hashimoto R, Tanaka T, Kudo T, Chiba S, Takeda M: Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch-1. Mol Cell Biol. 2008 Jan;28(1):165-76. [Abstract] [Full Text] Watanabe K, Nagaoka T, Lee JM, Bianco C, Gonzales M, Castro NP, Rangel MC, Sakamoto K, Sun Y, Callahan R, Salomon DS: Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1. J Cell Biol. 2009 Nov 2;187(3):343-53. [Abstract] [Full Text] Kefas B, Comeau L, Floyd DH, Seleverstov O, Godlewski J, Schmittgen T, Jiang J, diPierro CG, Li Y, Chiocca EA, Lee J, Fine H, Abounader R, Lawler S, Purow B: The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors. J Neurosci. 2009 Dec 2;29(48):15161-8. [Abstract] [Full Text] Espinosa L, Santos S, Inglés-Esteve J, Muñoz-Canoves P, Bigas A: p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR. J Cell Sci. 2002 Mar 15;115(Pt 6):1295-303. [Abstract] [Full Text] Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice. J Clin Invest. 2010 Jul 1;120(7):2537-48. [Abstract] [Full Text] Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J Cell Biol. 2007 May 21;177(4):647-57. [Abstract] [Full Text] *And many other.

Species Reactivity:

Human
Mouse

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Western Blotting
Immunohistochemistry (frozen sections)
Immunocytochemistry

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Huppert SS, Le A, Schroeter EH, Mumm JS, Saxena MT, Milner LA, Kopan R: Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1. Nature. 2000 Jun 22;405(6789):966-70. [Abstract]

*Tagami S, Okochi M, Yanagida K, Ikuta A, Fukumori A, Matsumoto N, Ishizuka-Katsura Y, Nakayama T, Itoh N, Jiang J, Nishitomi K, Kamino K, Morihara T, Hashimoto R, Tanaka T, Kudo T, Chiba S, Takeda M: Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch-1. Mol Cell Biol. 2008 Jan;28(1):165-76. [Abstract] [Full Text]

*Watanabe K, Nagaoka T, Lee JM, Bianco C, Gonzales M, Castro NP, Rangel MC, Sakamoto K, Sun Y, Callahan R, Salomon DS: Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1. J Cell Biol. 2009 Nov 2;187(3):343-53. [Abstract] [Full Text]

*Kefas B, Comeau L, Floyd DH, Seleverstov O, Godlewski J, Schmittgen T, Jiang J, diPierro CG, Li Y, Chiocca EA, Lee J, Fine H, Abounader R, Lawler S, Purow B: The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors. J Neurosci. 2009 Dec 2;29(48):15161-8. [Abstract] [Full Text]

Espinosa L, Santos S, Inglés-Esteve J, Muñoz-Canoves P, Bigas A: p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR. J Cell Sci. 2002 Mar 15;115(Pt 6):1295-303. [Abstract] [Full Text]

*Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice. J Clin Invest. 2010 Jul 1;120(7):2537-48. [Abstract] [Full Text]

*Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J Cell Biol. 2007 May 21;177(4):647-57. [Abstract] [Full Text]

*And many other.

For research use only. Not for drug, diagnostic or other use.

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