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Mouse Monoclonal to Notch 1

mN1A (IgG1)

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  11-651-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-651-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-651-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-651-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-651-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-651-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged1 (CD339), and Jagged2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.

Specificity

The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

Regulatory Status

Immunogen

GST fusion protein containing cdc10-NCR region of mouse Notch1

Species Reactivity:

  • Human
  • Mouse

Negative Species:

  • Rat

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Huppert SS, Le A, Schroeter EH, Mumm JS, Saxena MT, Milner LA, Kopan R: Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1. Nature. 2000 Jun 22;405(6789):966-70. [Abstract]
  • *Tagami S, Okochi M, Yanagida K, Ikuta A, Fukumori A, Matsumoto N, Ishizuka-Katsura Y, Nakayama T, Itoh N, Jiang J, Nishitomi K, Kamino K, Morihara T, Hashimoto R, Tanaka T, Kudo T, Chiba S, Takeda M: Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch-1. Mol Cell Biol. 2008 Jan;28(1):165-76. [Abstract] [Full Text]
  • *Watanabe K, Nagaoka T, Lee JM, Bianco C, Gonzales M, Castro NP, Rangel MC, Sakamoto K, Sun Y, Callahan R, Salomon DS: Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1. J Cell Biol. 2009 Nov 2;187(3):343-53. [Abstract] [Full Text]
  • *Kefas B, Comeau L, Floyd DH, Seleverstov O, Godlewski J, Schmittgen T, Jiang J, diPierro CG, Li Y, Chiocca EA, Lee J, Fine H, Abounader R, Lawler S, Purow B: The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors. J Neurosci. 2009 Dec 2;29(48):15161-8. [Abstract] [Full Text]
  • Espinosa L, Santos S, Inglés-Esteve J, Muñoz-Canoves P, Bigas A: p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR. J Cell Sci. 2002 Mar 15;115(Pt 6):1295-303. [Abstract] [Full Text]
  • *Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice. J Clin Invest. 2010 Jul 1;120(7):2537-48. [Abstract] [Full Text]
  • *Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J Cell Biol. 2007 May 21;177(4):647-57. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    mN1A purified

    Fig. 1. Intracellular staining of Notch1 in Jurkat cells using anti-Notch1 (mN1A) purified.


    mN1A APC

    Fig. 2. Intracellular staining of Notch1 in MOLT-4 cells using anti-Notch1 (mN1A) APC.


    1P-651

    Fig. 3. Intracellular staining of Notch1 in Jurkat cells using anti-Notch1 (mN1A) PE.

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