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Mouse Monoclonal to CD107a / LAMP-1

H4A3 (IgG1)

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  10-671-C025 azide free 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  PB-671-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
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  A4-671-T025 Alexa Fluor® 488 25 tests yes choose region PDF datasheetHTML datasheet buy
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  1P-671-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
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  1A-671-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-671-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  T9-671-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.

Specificity

The mouse monoclonal antibody H4A3 recognizes CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.

Regulatory Status

Immunogen

Human PBMC

Species Reactivity:

  • Human
  • Non-Human Primates
  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
  • Western Blotting
    Recommended dilution:1 μg/ml
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, August JT: Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 1989 Jan;268(1):360-78. [Abstract]
  • Furuta K, Ikeda M, Nakayama Y, Nakamura K, Tanaka M, Hamasaki N, Himeno M, Hamilton SR, August JT: Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases. Am J Pathol. 2001 Aug;159(2):449-55. [Abstract]
  • *Deetz CO, Hebbeler AM, Propp NA, Cairo C, Tikhonov I, Pauza CD: Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys. Infect Immun. 2006 Aug;74(8):4505-11. [Abstract] [Full Text]
  • *Marcenaro S, Gallo F, Martini S, Santoro A, Griffiths GM, Aricˇ M, Moretta L, Pende D: Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease. Blood. 2006 Oct 1;108(7):2316-23. [Abstract] [Full Text]
  • *Carlsten M, Bj÷rkstr÷m NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25. [Abstract] [Full Text]
  • *Tomescu C, Chehimi J, Maino VC, Montaner LJ: NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells. J Immunol. 2007 Aug 15;179(4):2097-104. [Abstract] [Full Text]
  • *Yu CI, Gallegos M, Marches F, Zurawski G, Ramilo O, GarcÝa-Sastre A, Banchereau J, Palucka AK: Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines. Blood. 2008 Nov 1;112(9):3671-8. [Abstract] [Full Text]
  • *Mao H, Tu W, Liu Y, Qin G, Zheng J, Chan PL, Lam KT, Peiris JS, Lau YL: Inhibition of human natural killer cell activity by influenza virions and hemagglutinin. J Virol. 2010 May;84(9):4148-57. [Abstract] [Full Text]
  • *Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    H4A3 PE


    Fig. 1. Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.


    H4A3 azide free


    Fig. 2. Intracellular staining of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.


    H4A3 APC


    Fig. 3. Intracellular staining of human peripheral blood cells with anti-CD107a (H4A3) APC.


    H4A3 AF488


    Fig. 4. Intracellular staining of human peripheral blood cells with anti-CD107a (H4A3) Alexa Fluor® 488.


    H4A3 PC55


    Fig. 5. Flow cytometry analysis of PHA-activated PBMC with anti-CD107a (H4A3) PerCP-CyTM5.5.

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