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Mouse Monoclonal to STRO-1

STRO-1 (IgM)

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  11-649-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1P-649-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1A-649-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.

Specificity

The mouse monoclonal antibody STRO-1 recognizes the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.

Regulatory Status

Immunogen

Human CD34 positive bone marrow cells

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Simmons PJ, Torok-Storb B: Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood. 1991 Jul 1;78(1):55-62. [Abstract] [Full Text]
  • *Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, Shi S: Recovery of stem cells from cryopreserved periodontal ligament. J Dent Res. 2005 Oct;84(10):907-12. [Abstract]
  • *Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A, Simmons PJ: Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci. 2003 May 1;116(Pt 9):1827-35. [Abstract] [Full Text]
  • *Stenderup K, Justesen J, Eriksen EF, Rattan SI, Kassem M: Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis. J Bone Miner Res. 2001 Jun;16(6):1120-9. [Abstract]
  • *Kaneko R, Akita H, Shimauchi H, Sasano Y: Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy. J Electron Microsc (Tokyo). 2009 Dec;58(6):363-73. [Abstract]
  • *Bensidhoum M, Chapel A, Francois S, Demarquay C, Mazurier C, Fouillard L, Bouchet S, Bertho JM, Gourmelon P, Aigueperse J, Charbord P, Gorin NC, Thierry D, Lopez M: Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment. Blood. 2004 May 1;103(9):3313-9. [Abstract] [Full Text]
  • *Oyajobi BO, Lomri A, Hott M, Marie PJ: Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1 monoclonal antibody. J Bone Miner Res. 1999 Mar;14(3):351-61. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    STRO-1
    Fig. 1.


    Fig. 1. Surface staining of Kg1a cells with anti-STRO-1 (STRO-1) purified.


    STRO-1 APC


    Fig. 2. Surface staining of human peripheral blood with anti-STRO-1 (STRO-1) APC.


    STRO-1 PE


    Fig. 3. Surface staining of human peripheral blood with anti-STRO-1 (STRO-1) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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