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Mouse Monoclonal to TCR alpha/beta (rat)

R73 (IgG1)

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  11-653-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-653-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-653-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-653-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-653-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Specificity

The mouse monoclonal R73 recognizes TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.

Regulatory Status

Immunogen

Rat T blasts and erythrocytes

Species Reactivity:

  • Non-Human Primates
  • Rat

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • Functional Application
    in vitro activation and in vivo depletion of TCR alpha/beta T cells
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Radojevic K, Arsenovic-Ranin N, Kosec D, Pesic V, Pilipovic I, Perisic M, Plecas-Solarovic B, Leposavic G: Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level. J Endocrinol. 2007 Mar;192(3):669-82. [Abstract] [Full Text]
  • *Ritter T, Schröder G, Risch K, Vergopoulos A, Shean MK, Kolls J, Brock J, Lehmann M, Volk HD: Ischemia/reperfusion injury-mediated down-regulation of adenovirus-mediated gene expression in a rat heart transplantation model is inhibited by co-application of a TNFRp55-Ig chimeric construct. Gene Ther. 2000 Jul;7(14):1238-43. [Abstract] [Full Text]
  • *Fuller JM, Bogdani M, Tupling TD, Jensen RA, Pefley R, Manavi S, Cort L, Blankenhorn EP, Mordes JP, Lernmark A, Kwitek AE: Genetic dissection reveals diabetes loci proximal to the gimap5 lymphopenia gene. Physiol Genomics. 2009 Jun 10;38(1):89-97. [Abstract] [Full Text]
  • *Hünig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G: A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989 Jan 1;169(1):73-86. [Abstract] [Full Text]
  • *Kreiss M, Asmuss A, Krejci K, Lindemann D, Miyoshi-Akiyama T, Uchiyama T, Rink L, Broeren CP, Herrmann T: Contrasting contributions of complementarity-determining region 2 and hypervariable region 4 of rat BV8S2+ (Vbeta8.2) TCR to the recognition of myelin basic protein and different types of bacterial superantigens. Int Immunol. 2004 May;16(5):655-63. [Abstract] [Full Text]
  • *Wilhelm MJ, Pratschke J, Beato F, Taal M, Kusaka M, Hancock WW, Tilney NL: Activation of the heart by donor brain death accelerates acute rejection after transplantation. Circulation. 2000 Nov 7;102(19):2426-33. [Abstract] [Full Text]
  • *Hansson AS, Heinegård D, Holmdahl R: A new animal model for relapsing polychondritis, induced by cartilage matrix protein (matrilin-1). J Clin Invest. 1999 Sep;104(5):589-98. [Abstract] [Full Text]
  • *Müller N, van den Brandt J, Odoardi F, Tischner D, Herath J, Flügel A, Reichardt HM: A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. J Clin Invest. 2008 Apr;118(4):1405-16. [Abstract] [Full Text]
  • *Li S, Thanikachalam M, Pang M, Kawaharada N, Aitouche A, Pham SM: A clinically relevant CTLA4-Ig-based regimen induces chimerism and tolerance to heart grafts. Ann Thorac Surg. 2001 Oct;72(4):1306-10. [Abstract]
  • *Shao H, Sun SL, Kaplan HJ, Sun D: Characterization of rat CD8+ uveitogenic T cells specific for interphotoreceptor retinal-binding protein 1177-1191: J Immunol. 2004 Aug 15;173(4):2849-54. [Abstract] [Full Text]
  • *Carlson BC, Jansson AM, Larsson A, Bucht A, Lorentzen JC: The endogenous adjuvant squalene can induce a chronic T-cell-mediated arthritis in rats. Am J Pathol. 2000 Jun;156(6):2057-65. [Abstract] [Full Text]
  • *Kerstan A, Hünig T: Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells. J Immunol. 2004 Feb 1;172(3):1341-5. [Abstract] [Full Text]
  • *Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26(11):2582-6. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    R73 PE FC


    Fig. 1. Surface staining of rat thymocytes with anti-rat TCR alpha/beta (R73) PE.


    R73 FITC


    Fig. 2. Surface staining of rat splenocytes with anti-rat TCR alpha/beta (R73) FITC.

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