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Mouse Monoclonal to CD11b

ICRF44 (IgG1)

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  11-681-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  PB-681-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
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  A4-681-T025 Alexa Fluor® 488 25 tests yes choose region PDF datasheetHTML datasheet buy
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  1P-681-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

Specificity

The mouse monoclonal antibody ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
HLDA IV, WS Code M047

Regulatory Status

Immunogen

Rheumatoid synovial cells and human monocytes.

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • Functional Application
    blocking of granulocyte activation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood. 2001 May 15;97(10):2932-40. [Abstract] [Full Text]
  • *Tieu BC, Lee C, Sun H, Lejeune W, Recinos A 3rd, Ju X, Spratt H, Guo DC, Milewicz D, Tilton RG, Brasier AR: An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice. J Clin Invest. 2009 Dec;119(12):3637-51. [Abstract] [Full Text]
  • *Masten BJ, Olson GK, Tarleton CA, Rund C, Schuyler M, Mehran R, Archibeque T, Lipscomb MF: Characterization of myeloid and plasmacytoid dendritic cells in human lung. J Immunol. 2006 Dec 1;177(11):7784-93. [Abstract] [Full Text]
  • *Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int Immunol. 2001 Dec;13(12):1571-81. [Abstract] [Full Text]
  • *Hua J, Sakamoto K, Nagaoka I: Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the functions of neutrophils. J Leukoc Biol. 2002 Apr;71(4):632-40. [Abstract] [Full Text]
  • *Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    ICRF44 APC-Cy7


    Fig. 1. Surface staining of human peripheral blood with anti-CD11b (ICRF44) APC-CyTM7.



    ICRF44 PE


    Fig. 2. Surface staining of human peripheral blood with anti-CD11b (ICRF44) PE.



    ICRF44 AF488


    Fig. 3. Surface staining of human peripheral blood with anti-CD11b (ICRF44) Alexa Fluor® 488.


    1F-681


    Fig. 4. Surface staining of human peripheral blood with anti-CD11b (ICRF44) FITC.

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