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Mouse Monoclonal to 5-bromodeoxyuridine (BrdU)

Bu20a (IgG1)

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Background

Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.

Specificity

The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining.

Regulatory Status

Immunogen

Bromodeoxyuridine conjugated to BSA

Species Reactivity:

Negative Species:

Applications:

  • Flow Cytometry
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem. 1989 Oct;37(10):1517-27. [Abstract] [Full Text]
  • O'Driscoll M, Jeggo PA: CsA can induce DNA double-strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair. Bone Marrow Transplant. 2008 Jun;41(11):983-9. [Abstract] [Full Text]
  • *Pajalunga D, Mazzola A, Salzano AM, Biferi MG, De Luca G, Crescenzi M: Critical requirement for cell cycle inhibitors in sustaining nonproliferative states. J Cell Biol. 2007 Mar 12;176(6):807-18. [Abstract] [Full Text]
  • *Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB: Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA. J Histochem Cytochem. 2003 Dec;51(12):1681-8. [Abstract] [Full Text]
  • *Lagares A, Li HY, Zhou XF, Avendaño C: Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. J Neurosci. 2007 Jul 25;27(30):7939-53. [Abstract] [Full Text]
  • *Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF: Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'-bromo-2'-deoxyuridine. Am J Physiol Cell Physiol. 2005 Sep;289(3):C757-65. [Abstract] [Full Text]
  • *Martín CA, Surur JM, García MN, Corrons F, Badrán AF: Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver. Biocell. 2005 Apr;29(1):33-8. [Abstract]
  • *Yatera K, Hsieh J, Hogg JC, Tranfield E, Suzuki H, Shih CH, Behzad AR, Vincent R, van Eeden SF: Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques. Am J Physiol Heart Circ Physiol. 2008 Feb;294(2):H944-53. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Bu20a PE


    Fig. 1. Intracellular staining of BrdU incorporated into the K562 cells. Stained using anti-Brdu (clone Bu20a) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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