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Mouse Monoclonal to Lambda light chains

1-155-2 (IgG1)

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Background

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

Specificity

The mouse monoclonal antibody 1-155-2 recognizes lambda light chains (22.5 kDa) of human immunoglobulin.

Regulatory Status

Immunogen

Human IgA1 lambda myeloma protein

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Kubagawa H, Gathings WE, Levitt D, Kearney JF, Cooper MD: Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence. J Clin Immunol. 1982 Oct;2(4):264-9. [Abstract]
  • *Tierens AM, Holte H, Warsame A, Ikonomou IM, Wang J, Chan WC, Delabie J: Low levels of monoclonal small B cells in the bone marrow of patients with diffuse large B-cell lymphoma of activated B-cell type but not of germinal center B-cell type. Haematologica. 2010 Aug;95(8):1334-41. [Abstract] [Full Text]
  • *Bahler DW, Pindzola JA, Swerdlow SH: Splenic marginal zone lymphomas appear to originate from different B cell types. Am J Pathol. 2002 Jul;161(1):81-8. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    1-155

    Fig. 1. Surface staining of lambda light chains in human peripheral blood with anti-lambda light chains (1-155-2) PE.


    1-155-2 APC-Cy7

    Fig. 2. Surface staining of lambda light chains in human peripheral blood with anti-lambda light chains (1-155-2) APC-CyTM7.


    1-155-2 APC

    Fig. 3. Surface staining of lambda light chains in human peripheral blood with anti-lambda light chains (1-155-2) APC.

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