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Mouse Monoclonal to CD99

3B2/TA8 (IgG2a)

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Background

CD99 is a ubiquitous transmembrane type I sialoglycoprotein of a unique and poorly characterized protein family. CD99 is heavily O-glycosylated and was described as a T cell costimulator and strong activator of integrin-mediated actin cytoskeleton assembly, promoting cell adhesion and homotypic aggregation, immediate arrest on an inflamed vascular endothelium, and cell migration through it. Ligation of CD99 under some conditions can lead to apoptosis. Originally CD99 was described as a human thymus leukemia antigen, an Ewing´s sarcoma-specific membrane marker, and an adhesion molecule involved in spontaneous rosette formation of T cells with erythrocytes.

Specificity

The mouse monoclonal antibody 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.
HLDA VI.; WS Code T 6T-097, BP 534

Regulatory Status

Immunogen

Human thymocytes

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Mass Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Waclavicek M, Majdic O, Stulnig T, Berger M, Sunder-Plassmann R, Zlabinger GJ, Baumruker T, Stöckl J, Ebner C, Knapp W, Pickl WF: CD99 engagement on human peripheral blood T cells results in TCR/CD3-dependent cellular activation and allows for Th1-restricted cytokine production. J Immunol. 1998 Nov 1;161(9):4671-8. [Abstract] [Full Text]
  • *Brémond A, Meynet O, Mahiddine K, Coito S, Tichet M, Scotlandi K, Breittmayer JP, Gounon P, Gleeson PA, Bernard A, Bernard G: Regulation of HLA class I surface expression requires CD99 and p230/golgin-245 interaction. Blood. 2009 Jan 8;113(2):347-57. [Abstract] [Full Text]
  • *Pickl WF, Pimentel-Muiños FX, Seed B: Lipid rafts and pseudotyping. J Virol. 2001 Aug;75(15):7175-83. [Abstract] [Full Text]
  • *Kueng HJ, Leb VM, Haiderer D, Raposo G, Thery C, Derdak SV, Schmetterer KG, Neunkirchner A, Sillaber C, Seed B, Pickl WF: General strategy for decoration of enveloped viruses with functionally active lipid-modified cytokines. J Virol. 2007 Aug;81(16):8666-76. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Fig. 1


    Fig. 1. Surface staining (mass cytometry) of human peripheral blood cells (after separation using Ficoll-Paque density gradient centrifugation) with anti-human CD99 (3B2/TA8) Eu153. Gated on singlets.


    3B2-TA8 APC


    Fig. 2. Surface staining of human peripheral blood cells with anti-human CD99 (3B2/TA8) APC.


    1p-658


    Fig. 3. Surface staining of human peripheral blood cells with anti-human CD99 (3B2/TA8) PE.

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