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by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies
categories: Apoptosis (Human)

Mouse Monoclonal to Bcl2

Bcl-2/100 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-668-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-668-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-668-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-668-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-668-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-668-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-668-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-668-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

Bcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.

Specificity

The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.

Regulatory Status

Immunogen

Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2

Species Reactivity:

  • Human

Negative Species:

  • Mouse

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Laforge M, Petit F, Estaquier J, Senik A: Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef. J Virol. 2007 Oct;81(20):11426-40. [Abstract] [Full Text]
  • *Laflamme C, IsraŽl-Assayag E, Cormier Y: Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis. Eur Respir J. 2003 Feb;21(2):225-31. [Abstract] [Full Text]
  • *Joubert A, Marais S, Maritz C: Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels. Biocell. 2009 Apr;33(1):67-70. [Abstract]
  • *Soilu-Hšnninen M, Ekert P, Bucci T, Syroid D, Bartlett PF, Kilpatrick TJ: Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway. J Neurosci. 1999 Jun 15;19(12):4828-38. [Abstract] [Full Text]
  • *Gugasyan R, Christou A, O'Reilly LA, Strasser A, Gerondakis S: Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA. Cell Death Differ. 2006 Jul;13(7):1235-7. [Abstract] [Full Text]
  • *And other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Bcl-2-100 PE


    Fig. 1. Intracellular staining of human peripheral blood with anti-Bcl2 (Bcl-2/100) PE.


    Bcl-2-100 FITC


    Fig. 2. Intracellular staining of human peripheral blood with anti-Bcl2 (Bcl-2/100) FITC.


    1A-668


    Fig. 3. Intracellular staining of human peripheral blood with anti-Bcl2 (Bcl-2/100) APC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIOīs term and conditions which are available at www.exbio.cz.
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