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Mouse Monoclonal to CD4 (rat)

OX-35 (IgG2a)

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  11-679-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-679-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-679-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-679-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-679-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-679-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-679-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell differenciation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

Specificity

The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).

Regulatory Status

Immunogen

MLR generated rat Th cells

Species Reactivity:

  • Rat

Negative Species:

Applications:

  • Flow Cytometry
  • Immunohistochemistry
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Dragun D, Lukitsch I, Tullius SG, Qun Y, Park JK, Schneider W, Luft FC, Haller H: Inhibition of intercellular adhesion molecule-1 with antisense deoxynucleotides prolongs renal isograft survival in the rat. Kidney Int. 1998 Dec;54(6):2113-22. [Abstract] [Full Text]
  • *Tsuji H, Kawaguchi S, Wada T, Nagoya S, Inobe M, Yamashita T, Ishii S, Uede T: Adenovirus-mediated in vivo B7-1 gene transfer induces anti-tumor immunity against pre-established primary tumor and pulmonary metastasis of rat osteosarcoma. Cancer Gene Ther. 2002 Sep;9(9):747-55. [Abstract] [Full Text]
  • *Salomon I, Netzer N, Wildbaum G, Schif-Zuck S, Maor G, Karin N: Targeting the function of IFN-gamma-inducible protein 10 suppresses ongoing adjuvant arthritis. J Immunol. 2002 Sep 1;169(5):2685-93. [Abstract] [Full Text]
  • *Yan Y, Devos T, Yu L, Xia G, Rutgeerts O, Goebels J, Segers C, Lin Y, Vandeputte M, Waer M: Pathogenesis of autoimmunity after xenogeneic thymus transplantation. J Immunol. 2003 Jun 15;170(12):5936-46. [Abstract] [Full Text]
  • *Hishikari K, Suzuki J, Ogawa M, Isobe K, Takahashi T, Onishi M, Takayama K, Isobe M: Pharmacological activation of the prostaglandin E2 receptor EP4 improves cardiac function after myocardial ischaemia/reperfusion injury. Cardiovasc Res. 2009 Jan 1;81(1):123-32. [Abstract] [Full Text]
  • *Gelderman KA, Hultqvist M, Holmberg J, Olofsson P, Holmdahl R: T cell surface redox levels determine T cell reactivity and arthritis susceptibility. Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12831-6. [Abstract] [Full Text]
  • *Ramiro-Puig E, Pérez-Cano FJ, Ramos-Romero S, Pérez-Berezo T, Castellote C, Permanyer J, Franch A, Izquierdo-Pulido M, Castell M: Intestinal immune system of young rats influenced by cocoa-enriched diet. J Nutr Biochem. 2008 Aug;19(8):555-65. [Abstract]
  • *Viel EC, Lemarié CA, Benkirane K, Paradis P, Schiffrin EL: Immune regulation and vascular inflammation in genetic hypertension. Am J Physiol Heart Circ Physiol. 2010 Mar;298(3):H938-44. [Abstract] [Full Text]
  • *Baba T, Iwasaki S, Maruoka T, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M, Ishizu A: Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/perforin-dependent mechanism. J Immunol. 2008 Mar 1;180(5):2999-3006. [Abstract] [Full Text]
  • *Baba T, Ishizu A, Iwasaki S, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M: CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype. Blood. 2006 Mar 1;107(5):2004-12. [Abstract] [Full Text]
  • *Monzon-Casanova E, Steiniger B, Schweigle S, Clemen H, Zdzieblo D, Starick L, Müller I, Wang CR, Rhost S, Cardell S, Pyz E, Herrmann T: CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation. PLoS One. 2010 Sep 30;5(9). pii: e13089. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    OX-35 FITC FC


    Fig. 1. Surface staining of rat thymocytes with anti-rat CD4 (OX-35) FITC.


    OX-35 PE FC


    Fig. 2. Surface staining of rat thymocytes with anti-rat CD4 (OX-35) PE.

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