categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD33

WM53 (IgG1)

Product No.	Form	Quantity	Price
11-662-C025	purified	0.025 mg	choose region
11-662-C100	purified	0.1 mg	choose region
12-662-C100	low endotoxin	0.1 mg	choose region
1F-662-T025	FITC	25 tests	choose region
1F-662-T100	FITC	100 tests	choose region
1P-662-T025	PE	25 tests	choose region
1P-662-T100	PE	100 tests	choose region
1A-662-T025	APC	25 tests	choose region
1A-662-T100	APC	100 tests	choose region
T8-662-T025	PE-Cy™5	25 tests	choose region
T8-662-T100	PE-Cy™5	100 tests	choose region
PC-662-T025	PerCP	25 tests	choose region
PC-662-T100	PerCP	100 tests	choose region
T9-662-T025	PerCP-Cy™5.5	25 tests	choose region
T9-662-T100	PerCP-Cy™5.5	100 tests	choose region
A7-662-T025	Alexa Fluor® 700	25 tests	choose region
A7-662-T100	Alexa Fluor® 700	100 tests	choose region

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Background

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.

Entrez Gene (human): 19q13.3

Specificity

The mouse monoclonal antibody WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
HLDA IV; WS Code M-505

Immunogen

Human AML cells

Species Reactivity: Human Non-Human Primates Negative Species:	Applications: Flow Cytometry Immunoprecipitation Western Blotting Immunohistochemistry (frozen sections) Application note: acetone fixation Immunocytochemistry Functional Application Induction of cytokine production Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC: Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 1987 Dec;5(4):215-25. [Abstract] Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Zowtyj H, Zola H: Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33. Br J Haematol. 1988 Jun;69(2):163-71. [Abstract] Bradstock KF, Kirk J, Grimsley PG, Kabral A, Hughes WG: Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations. Br J Haematol. 1989 Aug;72(4):512-8. [Abstract] Shin YK, Choi EY, Kim SH, Chung J, Chung DH, Park WS, Jung KC, Kim HS, Park S, Kim HJ, Park MH, Min CK, Kim CC, Park SH: Expression of leukemia-associated antigen, JL1, in bone marrow and thymus. Am J Pathol. 2001 Apr;158(4):1473-80. [Abstract] [Full Text] Vitale C, Romagnani C, Puccetti A, Olive D, Costello R, Chiossone L, Pitto A, Bacigalupo A, Moretta L, Mingari MC: Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc Natl Acad Sci U S A. 2001 May 8;98(10):5764-9. [Abstract] [Full Text] Hernández-López C, Varas A, Sacedón R, Jiménez E, Muñoz JJ, Zapata AG, Vicente A: Stromal cell-derived factor 1/CXCR4 signaling is critical for early human T-cell development. Blood. 2002 Jan 15;99(2):546-54. [Abstract] [Full Text] Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function. Eur Heart J. 2005 Jun;26(12):1196-204. [Abstract] [Full Text] Schenk M, Bouchon A, Birrer S, Colonna M, Mueller C: Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine. J Immunol. 2005 Jan 1;174(1):517-24. [Abstract] [Full Text] Garnache-Ottou F, Chaperot L, Biichle S, Ferrand C, Remy-Martin JP, Deconinck E, de Tailly PD, Bulabois B, Poulet J, Kuhlein E, Jacob MC, Salaun V, Arock M, Drenou B, Schillinger F, Seilles E, Tiberghien P, Bensa JC, Plumas J, Saas P: Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells. Blood. 2005 Feb 1;105(3):1256-64. [Abstract] [Full Text] Hernández-Caselles T, Martínez-Esparza M, Pérez-Oliva AB, Quintanilla-Cecconi AM, García-Alonso A, Alvarez-López DM, García-Peñarrubia P: A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J Leukoc Biol. 2006 Jan;79(1):46-58. [Abstract] [Full Text] *And many other.

Species Reactivity:

Human
Non-Human Primates

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Western Blotting
Immunohistochemistry (frozen sections)
Application note: acetone fixation
Immunocytochemistry
Functional Application
Induction of cytokine production

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC: Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 1987 Dec;5(4):215-25. [Abstract]

*Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Zowtyj H, Zola H: Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33. Br J Haematol. 1988 Jun;69(2):163-71. [Abstract]

*Bradstock KF, Kirk J, Grimsley PG, Kabral A, Hughes WG: Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations. Br J Haematol. 1989 Aug;72(4):512-8. [Abstract]

*Shin YK, Choi EY, Kim SH, Chung J, Chung DH, Park WS, Jung KC, Kim HS, Park S, Kim HJ, Park MH, Min CK, Kim CC, Park SH: Expression of leukemia-associated antigen, JL1, in bone marrow and thymus. Am J Pathol. 2001 Apr;158(4):1473-80. [Abstract] [Full Text]

*Vitale C, Romagnani C, Puccetti A, Olive D, Costello R, Chiossone L, Pitto A, Bacigalupo A, Moretta L, Mingari MC: Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc Natl Acad Sci U S A. 2001 May 8;98(10):5764-9. [Abstract] [Full Text]

*Hernández-López C, Varas A, Sacedón R, Jiménez E, Muñoz JJ, Zapata AG, Vicente A: Stromal cell-derived factor 1/CXCR4 signaling is critical for early human T-cell development. Blood. 2002 Jan 15;99(2):546-54. [Abstract] [Full Text]

*Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function. Eur Heart J. 2005 Jun;26(12):1196-204. [Abstract] [Full Text]

*Schenk M, Bouchon A, Birrer S, Colonna M, Mueller C: Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine. J Immunol. 2005 Jan 1;174(1):517-24. [Abstract] [Full Text]

*Garnache-Ottou F, Chaperot L, Biichle S, Ferrand C, Remy-Martin JP, Deconinck E, de Tailly PD, Bulabois B, Poulet J, Kuhlein E, Jacob MC, Salaun V, Arock M, Drenou B, Schillinger F, Seilles E, Tiberghien P, Bensa JC, Plumas J, Saas P: Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells. Blood. 2005 Feb 1;105(3):1256-64. [Abstract] [Full Text]

*Hernández-Caselles T, Martínez-Esparza M, Pérez-Oliva AB, Quintanilla-Cecconi AM, García-Alonso A, Alvarez-López DM, García-Peñarrubia P: A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J Leukoc Biol. 2006 Jan;79(1):46-58. [Abstract] [Full Text]

*And many other.

For research use only. Not for drug, diagnostic or other use.

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