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Mouse Monoclonal to CD33

WM53 (IgG1)

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  11-662-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-662-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  12-662-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-662-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-662-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-662-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-662-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-662-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-662-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  T8-662-T025 PE-Cy™5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T8-662-T100 PE-Cy™5 100 tests yes choose region PDF datasheetHTML datasheet buy
  PC-662-T025 PerCP 25 tests yes choose region PDF datasheetHTML datasheet buy
  PC-662-T100 PerCP 100 tests yes choose region PDF datasheetHTML datasheet buy
  T9-662-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T9-662-T100 PerCP-Cy™5.5 100 tests yes choose region PDF datasheetHTML datasheet buy
  A7-662-T025 Alexa Fluor® 700 25 tests yes choose region PDF datasheetHTML datasheet buy
  A7-662-T100 Alexa Fluor® 700 100 tests yes choose region PDF datasheetHTML datasheet buy
  T7-662-T100 PE-Cy™7 100 tests yes choose region PDF datasheetHTML datasheet buy
  T4-662-T025 APC-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
  T4-662-T100 APC-Cy™7 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.

Specificity

The mouse monoclonal antibody WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
HLDA IV; WS Code M-505

Regulatory Status

Immunogen

Human AML cells

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (frozen sections)
    Application note: acetone fixation
  • Immunocytochemistry
  • Mass Cytometry
  • Functional Application
    Induction of cytokine production
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC: Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 1987 Dec;5(4):215-25. [Abstract]
  • *Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Zowtyj H, Zola H: Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33. Br J Haematol. 1988 Jun;69(2):163-71. [Abstract]
  • *Bradstock KF, Kirk J, Grimsley PG, Kabral A, Hughes WG: Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations. Br J Haematol. 1989 Aug;72(4):512-8. [Abstract]
  • *Shin YK, Choi EY, Kim SH, Chung J, Chung DH, Park WS, Jung KC, Kim HS, Park S, Kim HJ, Park MH, Min CK, Kim CC, Park SH: Expression of leukemia-associated antigen, JL1, in bone marrow and thymus. Am J Pathol. 2001 Apr;158(4):1473-80. [Abstract] [Full Text]
  • *Vitale C, Romagnani C, Puccetti A, Olive D, Costello R, Chiossone L, Pitto A, Bacigalupo A, Moretta L, Mingari MC: Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc Natl Acad Sci U S A. 2001 May 8;98(10):5764-9. [Abstract] [Full Text]
  • *Hernández-López C, Varas A, Sacedón R, Jiménez E, Muñoz JJ, Zapata AG, Vicente A: Stromal cell-derived factor 1/CXCR4 signaling is critical for early human T-cell development. Blood. 2002 Jan 15;99(2):546-54. [Abstract] [Full Text]
  • *Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function. Eur Heart J. 2005 Jun;26(12):1196-204. [Abstract] [Full Text]
  • *Schenk M, Bouchon A, Birrer S, Colonna M, Mueller C: Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine. J Immunol. 2005 Jan 1;174(1):517-24. [Abstract] [Full Text]
  • *Garnache-Ottou F, Chaperot L, Biichle S, Ferrand C, Remy-Martin JP, Deconinck E, de Tailly PD, Bulabois B, Poulet J, Kuhlein E, Jacob MC, Salaun V, Arock M, Drenou B, Schillinger F, Seilles E, Tiberghien P, Bensa JC, Plumas J, Saas P: Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells. Blood. 2005 Feb 1;105(3):1256-64. [Abstract] [Full Text]
  • *Hernández-Caselles T, Martínez-Esparza M, Pérez-Oliva AB, Quintanilla-Cecconi AM, García-Alonso A, Alvarez-López DM, García-Peñarrubia P: A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J Leukoc Biol. 2006 Jan;79(1):46-58. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    WM53 PE-Cy5


    Fig. 1. Surface staining of human peripheral blood with anti-CD33 (WM53) PE-CyTM5.


    WM53 purified MC

    Fig. 2. Surface staining (mass cytometry) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.


    WM53 APC-Cy7


    Fig. 3. Surface staining of human peripheral blood with anti-CD33 (WM53) APC-CyTM7.


    WM53 PE


    Fig. 4. Surface staining of human peripheral blood with anti-CD33 (WM53) PE.


    WM53 PC55


    Fig. 5. Surface staining of human peripheral blood with anti-CD33 (WM53) PerCP-CyTM5.5.


    WM53 APC


    Fig. 6. Surface staining of human peripheral blood with anti-CD33 (WM53) APC.


    1F-662


    Fig. 7. Surface staining of human peripheral blood with anti-CD33 (WM53) FITC.

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