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Mouse Monoclonal to CD62P / P-selectin

AK4 (IgG1)

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  11-667-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  12-667-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-667-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-667-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-667-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-667-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-667-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-667-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-667-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
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  T8-667-T025 PE-Cy™5 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.

Specificity

The antibody AK4 recognizes CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.
HLDA VI; WS Code P-44

Regulatory Status

Immunogen

Human platelets

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1.5 μg/ml
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
  • Functional Application
    Blocking
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Skinner MP, Lucas CM, Burns GF, Chesterman CN, Berndt MC: GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem. 1991 Mar 25;266(9):5371-4. [Abstract] [Full Text]
  • *Dunlop LC, Skinner MP, Bendall LJ, Favaloro EJ, Castaldi PA, Gorman JJ, Gamble JR, Vadas MA, Berndt MC: Characterization of GMP-140 (P-selectin) as a circulating plasma protein. J Exp Med. 1992 Apr 1;175(4):1147-50. [Abstract] [Full Text]
  • *Holme PA, Müller F, Solum NO, Brosstad F, Frøland SS, Aukrust P: Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection. FASEB J. 1998 Jan;12(1):79-89. [Abstract] [Full Text]
  • *Kowalska MA, Ratajczak J, Hoxie J, Brass LF, Gewirtz A, Poncz M, Ratajczak MZ: Megakaryocyte precursors, megakaryocytes and platelets express the HIV co-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets. Br J Haematol. 1999 Feb;104(2):220-9. [Abstract]
  • *Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, Baatz H, Kaufmann R, von Andrian UH, Zollner TM: Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J Invest Dermatol. 2004 Mar;122(3):830-6. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    AK4 APC


    Fig. 1. Surface staining of human peripheral blood with anti-CD62P (AK4) APC.



    AK4


    Fig. 2. Surface staining of human peripheral blood with anti-CD62P (AK4) PE.




    1B-667


    Fig. 3. Surface staining of human peripheral blood with anti-CD62P (AK4) biotin.


    1F-667


    Fig. 4. Surface staining of human peripheral blood with anti-CD62P (AK4) FITC.



    AK4 PE-Cy5


    Fig. 5. Surface staining of human peripheral blood with anti-CD62P (AK4) PE-CyTM5.

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