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Mouse Monoclonal to CD73

AD2 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-675-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-675-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-675-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-675-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-675-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-675-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.

Specificity

The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on T and B cell subsets, follicular dendritic cells and endothelial cells.

Regulatory Status

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Mass Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Borrione P, Peola S, Mariani S, Besostri B, Mallone R, Malavasi F, Pileri A, Massaia M: CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression. J Immunol. 1999 May 15;162(10):6238-46. [Abstract] [Full Text]
  • *Rodriguez MW, Paquet AC, Yang YH, Erle DJ: Differential gene expression by integrin beta 7+ and beta 7- memory T helper cells. BMC Immunol. 2004 Jul 5;5:13. [Abstract] [Full Text]
  • *Kögler G, Sensken S, Airey JA, Trapp T, Müschen M, Feldhahn N, Liedtke S, Sorg RV, Fischer J, Rosenbaum C, Greschat S, Knipper A, Bender J, Degistirici O, Gao J, Caplan AI, Colletti EJ, Almeida-Porada G, Müller HW, Zanjani E, Wernet P: A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med. 2004 Jul 19;200(2):123-35. [Abstract] [Full Text]
  • *Mokry J, Soukup T, Micuda S, Karbanova J, Visek B, Brcakova E, Suchanek J, Bouchal J, Vokurkova D, Ivancakova R: Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells. J Biomed Biotechnol. 2010;2010:673513. [Abstract] [Full Text]
  • *Nemoto E, Tada H, Shimauchi H: Disruption of CD40/CD40 ligand interaction with cleavage of CD40 on human gingival fibroblasts by human leukocyte elastase resulting in down-regulation of chemokine production. J Leukoc Biol. 2002 Sep;72(3):538-45. [Abstract] [Full Text]
  • *Semenov OV, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch AH, Malek A: Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation. Am J Obstet Gynecol. 2010 Feb;202(2):193 [Abstract]
  • *Hashikawa T, Takedachi M, Terakura M, Saho T, Yamada S, Thompson LF, Shimabukuro Y, Murakami S: Involvement of CD73 (ecto-5'-nucleotidase) in adenosine generation by human gingival fibroblasts. J Dent Res. 2003 Nov;82(11):888-92. [Abstract] [Full Text]
  • *Paquet-Fifield S, Schlüter H, Li A, Aitken T, Gangatirkar P, Blashki D, Koelmeyer R, Pouliot N, Palatsides M, Ellis S, Brouard N, Zannettino A, Saunders N, Thompson N, Li J, Kaur P: A role for pericytes as microenvironmental regulators of human skin tissue regeneration. J Clin Invest. 2009 Sep;119(9):2795-806. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    CD73

    Fig. 1. Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD73 (AD2) Yb176.


    AD2 FITC

    Fig. 2. Surface staining (flow cytometry) of human peripheral blood with anti-human CD73 (AD2) FITC. 



    AD2 PE

    Fig. 3. Surface staining (flow cytometry) of human peripheral blood with anti-human CD73 (AD2) PE. 

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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