categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD314

1D11 (IgG1)

Product No.	Form	Quantity	Price
11-650-C025	purified	0.025 mg	choose region
11-650-C100	purified	0.1 mg	choose region
10-650-C025	azide free	0.025 mg	choose region
10-650-C100	azide free	0.1 mg	choose region
1F-650-T025	FITC	25 tests	choose region
1F-650-T100	FITC	100 tests	choose region
1A-650-T025	APC	25 tests	choose region
1A-650-T100	APC	100 tests	choose region

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Background

CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.

Entrez Gene: 12p13.2-p12.3

Specificity

The mouse monoclonal antibody 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.

Immunogen

NKL cell line

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Immunoprecipitation Immunohistochemistry (frozen sections) Functional Application blocking of ligand binding Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Bauer S, Groh V, Wu J, Steinle A, Phillips JH, Lanier LL, Spies T: Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 1999 Jul 30;285(5428):727-9. [Abstract] Wu J, Cherwinski H, Spies T, Phillips JH, Lanier LL: DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells. J Exp Med. 2000 Oct 2;192(7):1059-68. [Abstract] [Full Text] Wu J, Groh V, Spies T: T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial gamma delta T cells. J Immunol. 2002 Aug 1;169(3):1236-40. [Abstract] [Full Text] Sangiolo D, Martinuzzi E, Todorovic M, Vitaggio K, Vallario A, Jordaney N, Carnevale-Schianca F, Capaldi A, Geuna M, Casorzo L, Nash RA, Aglietta M, Cignetti A: Alloreactivity and anti-tumor activity segregate within two distinct subsets of cytokine-induced killer (CIK) cells: implications for their infusion across major HLA barriers. Int Immunol. 2008 Jul;20(7):841-8. [Abstract] [Full Text] Hasenkamp J, Borgerding A, Uhrberg M, Falk C, Chapuy B, Wulf G, Jung W, Trümper L, Glass B: Self-tolerance of human natural killer cells lacking self-HLA-specific inhibitory receptors. Scand J Immunol. 2008 Mar;67(3):218-29. [Abstract] [Full Text] Ebert LM, Meuter S, Moser B: Homing and function of human skin gammadelta T cells and NK cells: relevance for tumor surveillance. J Immunol. 2006 Apr 1;176(7):4331-6. [Abstract] [Full Text] *Valencia J, Hernández-López C, Martínez VG, Hidalgo L, Zapata AG, Vicente A, Varas A, Sacedón R: Transient beta-catenin stabilization modifies lineage output from human thymic CD34+CD1a- progenitors. J Leukoc Biol. 2010 Mar;87(3):405-14. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Immunohistochemistry (frozen sections)
Functional Application
blocking of ligand binding

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Bauer S, Groh V, Wu J, Steinle A, Phillips JH, Lanier LL, Spies T: Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 1999 Jul 30;285(5428):727-9. [Abstract]

*Wu J, Cherwinski H, Spies T, Phillips JH, Lanier LL: DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells. J Exp Med. 2000 Oct 2;192(7):1059-68. [Abstract] [Full Text]

*Wu J, Groh V, Spies T: T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial gamma delta T cells. J Immunol. 2002 Aug 1;169(3):1236-40. [Abstract] [Full Text]

*Sangiolo D, Martinuzzi E, Todorovic M, Vitaggio K, Vallario A, Jordaney N, Carnevale-Schianca F, Capaldi A, Geuna M, Casorzo L, Nash RA, Aglietta M, Cignetti A: Alloreactivity and anti-tumor activity segregate within two distinct subsets of cytokine-induced killer (CIK) cells: implications for their infusion across major HLA barriers. Int Immunol. 2008 Jul;20(7):841-8. [Abstract] [Full Text]

*Hasenkamp J, Borgerding A, Uhrberg M, Falk C, Chapuy B, Wulf G, Jung W, Trümper L, Glass B: Self-tolerance of human natural killer cells lacking self-HLA-specific inhibitory receptors. Scand J Immunol. 2008 Mar;67(3):218-29. [Abstract] [Full Text]

*Ebert LM, Meuter S, Moser B: Homing and function of human skin gammadelta T cells and NK cells: relevance for tumor surveillance. J Immunol. 2006 Apr 1;176(7):4331-6. [Abstract] [Full Text]

*Valencia J, Hernández-López C, Martínez VG, Hidalgo L, Zapata AG, Vicente A, Varas A, Sacedón R: Transient beta-catenin stabilization modifies lineage output from human thymic CD34+CD1a- progenitors. J Leukoc Biol. 2010 Mar;87(3):405-14. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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