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Mouse Monoclonal to CD314

1D11 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-650-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-650-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  10-650-C025 azide free 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  10-650-C100 azide free 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-650-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-650-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-650-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-650-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-650-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-650-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.

Specificity

The mouse monoclonal antibody 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.

Regulatory Status

Immunogen

NKL cell line

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Functional Application
    blocking of ligand binding
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Bauer S, Groh V, Wu J, Steinle A, Phillips JH, Lanier LL, Spies T: Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 1999 Jul 30;285(5428):727-9. [Abstract]
  • *Wu J, Cherwinski H, Spies T, Phillips JH, Lanier LL: DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells. J Exp Med. 2000 Oct 2;192(7):1059-68. [Abstract] [Full Text]
  • *Wu J, Groh V, Spies T: T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial gamma delta T cells. J Immunol. 2002 Aug 1;169(3):1236-40. [Abstract] [Full Text]
  • *Sangiolo D, Martinuzzi E, Todorovic M, Vitaggio K, Vallario A, Jordaney N, Carnevale-Schianca F, Capaldi A, Geuna M, Casorzo L, Nash RA, Aglietta M, Cignetti A: Alloreactivity and anti-tumor activity segregate within two distinct subsets of cytokine-induced killer (CIK) cells: implications for their infusion across major HLA barriers. Int Immunol. 2008 Jul;20(7):841-8. [Abstract] [Full Text]
  • *Hasenkamp J, Borgerding A, Uhrberg M, Falk C, Chapuy B, Wulf G, Jung W, Trümper L, Glass B: Self-tolerance of human natural killer cells lacking self-HLA-specific inhibitory receptors. Scand J Immunol. 2008 Mar;67(3):218-29. [Abstract] [Full Text]
  • *Ebert LM, Meuter S, Moser B: Homing and function of human skin gammadelta T cells and NK cells: relevance for tumor surveillance. J Immunol. 2006 Apr 1;176(7):4331-6. [Abstract] [Full Text]
  • *Valencia J, Hernández-López C, Martínez VG, Hidalgo L, Zapata AG, Vicente A, Varas A, Sacedón R: Transient beta-catenin stabilization modifies lineage output from human thymic CD34+CD1a- progenitors. J Leukoc Biol. 2010 Mar;87(3):405-14. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    1D11 FITC


    Fig. 1. Surface staining of human peripheral blood with anti-human CD314 (1D11) FITC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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