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Mouse Monoclonal to EGFR

EGFR1 (IgG2b)

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  11-680-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1P-680-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
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  T7-680-T025 PE-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.

Specificity

The mouse monoclonal antibody EGFR1 reacts with extracellular domain of human protein kinase EGFR (ErbB1 / HER1); epitope within amino acids 6-273.

Regulatory Status

Immunogen

Human epidermoid carcinoma line A431

Species Reactivity:

  • Human
  • Equine (Horse)

Negative Species:

  • Mouse

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Aigner A, Juhl H, Malerczyk C, Tkybusch A, Benz CC, Czubayko F: Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation. Oncogene. 2001 Apr 19;20(17):2101-11. [Abstract] [Full Text]
  • *Akiyama M, Smith LT, Shimizu H: Changing patterns of localization of putative stem cells in developing human hair follicles. J Invest Dermatol. 2000 Feb;114(2):321-7. [Abstract] [Full Text]
  • *Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936. [Abstract] [Full Text]
  • *Fortunel NO, Hatzfeld JA, Rosemary PA, Ferraris C, Monier MN, Haydont V, Longuet J, Brethon B, Lim B, Castiel I, Schmidt R, Hatzfeld A: Long-term expansion of human functional epidermal precursor cells: promotion of extensive amplification by low TGF-beta1 concentrations. J Cell Sci. 2003 Oct 1;116(Pt 19):4043-52. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    EGFR1 PE-Cy7


    Fig. 1. Surface staining of EGFR on A431 cells with anti-EGFR (EGFR1) PE-CyTM7.


    EGFR PE


    Fig. 2. Surface staining of EGFR on A431 cells with anti-EGFR (EGFR1) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
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    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
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