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Mouse Monoclonal to CD34

581 (IgG1)

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  11-664-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1F-664-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-664-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-664-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
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  T8-664-T025 PE-Cy™5 25 tests yes choose region PDF datasheetHTML datasheet buy
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  PC-664-T025 PerCP 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Specificity

The mouse monoclonal antibody 581 reacts with CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.
HLDA V.; WS Code MA27

Regulatory Status

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:5 μg/ml
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Ando K, Nakamura Y, Chargui J, Matsuzawa H, Tsuji T, Kato S, Hotta T: Extensive generation of human cord blood CD34(+) stem cells from Lin(-)CD34(-) cells in a long-term in vitro system. Exp Hematol. 2000 Jun;28(6):690-9. [Abstract]
  • *Janowska-Wieczorek A, Marquez LA, Nabholtz JM, Cabuhat ML, Montaño J, Chang H, Rozmus J, Russell JA, Edwards DR, Turner AR: Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane. Blood. 1999 May 15;93(10):3379-90. [Abstract] [Full Text]
  • *Felschow DM, McVeigh ML, Hoehn GT, Civin CI, Fackler MJ: The adapter protein CrkL associates with CD34. Blood. 2001 Jun 15;97(12):3768-75. [Abstract] [Full Text]
  • *Kato S, Ando K, Nakamura Y, Muguruma Y, Sato T, Yabe H, Yabe M, Hattori K, Yasuda Y, Hotta T: Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation. Bone Marrow Transplant. 2001 Sep;28(6):587-95. [Abstract] [Full Text]
  • *Suárez L, Vidriales MB, García-Laraña J, Sanz G, Moreno MJ, López A, Barrena S, Martínez R, Tormo M, Palomera L, Lavilla E, López-Berges MC, de Santiago M, de Equiza ME, Miguel JF, Orfao A: CD34+ cells from acute myeloid leukemia, myelodysplastic syndromes, and normal bone marrow display different apoptosis and drug resistance-associated phenotypes. Clin Cancer Res. 2004 Nov 15;10(22):7599-606. [Abstract] [Full Text]
  • *Ono F, Sharma BK, Smith CC, Burnett JW, Aurelian L: CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM).J Invest Dermatol. 2005 Jun;124(6):1215-24. [Abstract] [Full Text]
  • *Ninos JM, Jefferies LC, Cogle CR, Kerr WG: The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells. J Transl Med. 2006 Feb 16;4:9. [Abstract] [Full Text]
  • *Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, Sadamoto K, Horii M, Matsumoto T, Murasawa S, Shibata T, Suehiro S, Asahara T: Dose-dependent contribution of CD34-positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery after myocardial infarction. Circulation. 2006 Mar 14;113(10):1311-25. [Abstract] [Full Text]
  • *Goardon N, Nikolousis E, Sternberg A, Chu WK, Craddock C, Richardson P, Benson R, Drayson M, Standen G, Vyas P, Freeman S: Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes. Haematologica. 2009 Aug;94(8):1160-3. [Abstract] [Full Text]
  • *Sanz E, Muñoz-A N, Monserrat J, Van-Den-Rym A, Escoll P, Ranz I, Alvarez-Mon M, de-la-Hera A: Ordering human CD34+CD10-CD19+ pre/pro-B-cell and CD19- common lymphoid progenitor stages in two pro-B-cell development pathways. Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5925-30. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    581 PE-Cy5

    Fig. 1. Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (581) PE-CyTM5.

    581 PE

    Fig. 2. Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (581) PE.

    581 PerCP-Cy5-5

    Fig. 3. Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (581) PerCP-CyTM5.5.

    581 purified

    Fig. 4. Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (581) purified.

    581 PerCP

    Fig. 5. Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (581) PerCP.

    581 APC

    Fig. 6. Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (581) APC.


    581 FITC

    Fig. 7. Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (581) FITC.

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