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Mouse Monoclonal to CD163

GHI/61 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-645-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-645-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-645-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-645-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-645-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-645-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  T9-645-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T9-645-T100 PerCP-Cy™5.5 100 tests yes choose region PDF datasheetHTML datasheet buy
  T7-645-T025 PE-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
  T7-645-T100 PE-Cy™7 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD163, also known as M130, is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.

Specificity

The mouse monoclonal antibody GHI/61 recognizes CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex.
HLDA VI; WS Code M38

Regulatory Status

Immunogen

Hairy cell leukemia cells

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY: A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4. Immunology. 1992 Apr;75(4):588-95. [Abstract] [Full Text]
  • *Law SK, Micklem KJ, Shaw JM, Zhang XP, Dong Y, Willis AC, Mason DY: A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. Eur J Immunol. 1993 Sep;23(9):2320-5. [Abstract]
  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Møller HJ, Peterslund NA, Graversen JH, Moestrup SK: Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma. Blood. 2002 Jan 1;99(1):378-80. [Abstract] [Full Text]
  • *Madsen M, Møller HJ, Nielsen MJ, Jacobsen C, Graversen JH, van den Berg T, Moestrup SK: Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region. J Biol Chem. 2004 Dec 3;279(49):51561-7. [Abstract] [Full Text]
  • *Philippidis P, Mason JC, Evans BJ, Nadra I, Taylor KM, Haskard DO, Landis RC: Hemoglobin scavenger receptor CD163 mediates interleukin-10 release and heme oxygenase-1 synthesis: antiinflammatory monocyte-macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery. Circ Res. 2004 Jan 9;94(1):119-26. [Abstract] [Full Text]
  • *Kim WK, Alvarez X, Fisher J, Bronfin B, Westmoreland S, McLaurin J, Williams K: CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood. Am J Pathol. 2006 Mar;168(3):822-34. [Abstract] [Full Text]
  • *Moniuszko M, Kowal K, Rusak M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin Vaccine Immunol. 2006 Jun;13(6):704-7. [Abstract] [Full Text]
  • *Bover LC, Cardˇ-Vila M, Kuniyasu A, Sun J, Rangel R, Takeya M, Aggarwal BB, Arap W, Pasqualini R: A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications. J Immunol. 2007 Jun 15;178(12):8183-94. [Abstract] [Full Text]
  • *Kusi KA, Gyan BA, Goka BQ, Dodoo D, Obeng-Adjei G, Troye-Blomberg M, Akanmori BD, Adjimani JP: Levels of soluble CD163 and severity of malaria in children in Ghana. Clin Vaccine Immunol. 2008 Sep;15(9):1456-60. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    CD163 APC
    Fig. 1.



    Fig. 1.
    Surface staining of human peripheral blood using anti-human CD163 (clone GHI/61) APC. CD163 was detected on CD14+ monocytes, whereas CD14- lymphocytes were used as a negative control.


    GHI-61 PE

    Fig. 2. Surface staining of human peripheral blood using anti-human CD163 (clone GHI/61) PE.



    GHI-64 PC7

    Fig. 3. Surface staining of human peripheral blood using anti-human CD163 (clone GHI/61) PE-CyTM7.


    T9-645

    Fig. 4. Surface staining of human peripheral blood using anti-human CD163 (clone GHI/61) PerCP-CyTM5.5.

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