categories: CD and Related Antigens & MHC (Rodent)

Mouse Monoclonal to TCR gamma/delta (rat)

V65 (IgG1)

Product No.	Form	Quantity	Price
11-654-C100	purified	0.1 mg	choose region
12-654-C500	low endotoxin	0.5 mg	choose region
1P-654-C100	PE	0.1 mg	choose region
1P-654-C500	PE	0.5 mg	choose region

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Background

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Specificity

The mouse monoclonal antibody V65 recognizes TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.

Immunogen

TCR alpha/beta-negative CD3-positive rat T cell hybridoma III.89.1.4 line

Species Reactivity: Rat Negative Species:	Applications: Flow Cytometry Recommended dilution:1 μg/ml Immunoprecipitation Immunohistochemistry (frozen sections) Functional Application in vitro activation, in vivo depletion of gamma/delta T cells Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Giezeman-Smits KM, Jonges LE, Chambers WH, Brisette-Storkus CS, Van Vlierberghe RL, Van Eendenburg JD, Eggermont AM, Fleuren GJ, Kuppen PJ: Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells. J Leukoc Biol. 1998 Feb;63(2):209-15. [Abstract] [Full Text] Hosea HJ, Rector ES, Taylor CG: Zinc-deficient rats have fewer recent thymic emigrant (CD90+) T lymphocytes in spleen and blood. J Nutr. 2003 Dec;133(12):4239-42. [Abstract] [Full Text] Tam FW, Smith J, Morel D, Karkar AM, Thompson EM, Cook HT, Pusey CD: Development of scarring and renal failure in a rat model of crescentic glomerulonephritis. Nephrol Dial Transplant. 1999 Jul;14(7):1658-66. [Abstract] [Full Text] Henriksson G, Helgeland L, Midtvedt T, Stierna P, Brandtzaeg P: Immune response to Mycoplasma pulmonis in nasal mucosa is modulated by the normal microbiota. Am J Respir Cell Mol Biol. 2004 Dec;31(6):657-62. [Abstract] [Full Text] Xystrakis E, Dejean AS, Bernard I, Druet P, Liblau R, Gonzalez-Dunia D, Saoudi A: Identification of a novel natural regulatory CD8 T-cell subset and analysis of its mechanism of regulation. Blood. 2004 Nov 15;104(10):3294-301. [Abstract] [Full Text] Moghaddami M, Cleland LG, Mayrhofer G: MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro. Int Immunol. 2005 Aug;17(8):1103-15. [Abstract] [Full Text] van den Brandt J, Kwon SH, Hünig T, McPherson KG, Reichardt HM: Sustained pre-TCR expression in Notch1IC-transgenic rats impairs T cell maturation and selection. J Immunol. 2005 Jun 15;174(12):7845-52. [Abstract] [Full Text] De Bernardis F, Lucciarini R, Boccanera M, Amantini C, Arancia S, Morrone S, Mosca M, Cassone A, Santoni G: Phenotypic and functional characterization of vaginal dendritic cells in a rat model of Candida albicans vaginitis. Infect Immun. 2006 Jul;74(7):4282-94. [Abstract] [Full Text] And many other.

Species Reactivity:

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:1 μg/ml
Immunoprecipitation
Immunohistochemistry (frozen sections)
Functional Application
in vitro activation, in vivo depletion of gamma/delta T cells

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Giezeman-Smits KM, Jonges LE, Chambers WH, Brisette-Storkus CS, Van Vlierberghe RL, Van Eendenburg JD, Eggermont AM, Fleuren GJ, Kuppen PJ: Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells. J Leukoc Biol. 1998 Feb;63(2):209-15. [Abstract] [Full Text]

*Hosea HJ, Rector ES, Taylor CG: Zinc-deficient rats have fewer recent thymic emigrant (CD90+) T lymphocytes in spleen and blood. J Nutr. 2003 Dec;133(12):4239-42. [Abstract] [Full Text]

*Tam FW, Smith J, Morel D, Karkar AM, Thompson EM, Cook HT, Pusey CD: Development of scarring and renal failure in a rat model of crescentic glomerulonephritis. Nephrol Dial Transplant. 1999 Jul;14(7):1658-66. [Abstract] [Full Text]

*Henriksson G, Helgeland L, Midtvedt T, Stierna P, Brandtzaeg P: Immune response to Mycoplasma pulmonis in nasal mucosa is modulated by the normal microbiota. Am J Respir Cell Mol Biol. 2004 Dec;31(6):657-62. [Abstract] [Full Text]

Xystrakis E, Dejean AS, Bernard I, Druet P, Liblau R, Gonzalez-Dunia D, Saoudi A: Identification of a novel natural regulatory CD8 T-cell subset and analysis of its mechanism of regulation. Blood. 2004 Nov 15;104(10):3294-301. [Abstract] [Full Text]

*Moghaddami M, Cleland LG, Mayrhofer G: MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro. Int Immunol. 2005 Aug;17(8):1103-15. [Abstract] [Full Text]

*van den Brandt J, Kwon SH, Hünig T, McPherson KG, Reichardt HM: Sustained pre-TCR expression in Notch1IC-transgenic rats impairs T cell maturation and selection. J Immunol. 2005 Jun 15;174(12):7845-52. [Abstract] [Full Text]

*De Bernardis F, Lucciarini R, Boccanera M, Amantini C, Arancia S, Morrone S, Mosca M, Cassone A, Santoni G: Phenotypic and functional characterization of vaginal dendritic cells in a rat model of Candida albicans vaginitis. Infect Immun. 2006 Jul;74(7):4282-94. [Abstract] [Full Text]

*And many other.

For research use only. Not for drug, diagnostic or other use.

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