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Mouse Monoclonal to CD8b (rat)

341 (IgG1)

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Background

The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Specificity

The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor, expressed on T cell subsets and some other cell types, such as macrophages.

Regulatory Status

Immunogen

CD8 positive Wistar rat splenic T cell hybridomas

Species Reactivity:

  • Rat

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1.5 μg/ml
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (frozen sections)
  • Functional Application
    Macrophage stimulation, in vitro blocking of allogenic responses
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26(11):2582-6. [Abstract]
  • *Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22(11):2841-8. [Abstract]
  • *Kühnlein P, Park JH, Herrmann T, Elbe A, Hünig T: Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994 Aug 1;153(3):979-86. [Abstract]
  • *Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158(4):1833-40. [Abstract]
  • *Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD: Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol. 1998 Jun 15;160(12):6004-11. [Abstract] [Full Text]
  • *Lin TJ, Hirji N, Nohara O, Stenton GR, Gilchrist M, Befus AD: Mast cells express novel CD8 molecules that selectively modulate mediator secretion. J Immunol. 1998 Dec 1;161(11):6265-72. [Abstract] [Full Text]
  • *Ellerman KE, Like AA: Islet cell membrane antigens activate diabetogenic CD4+ T-cells in the BB/Wor rat. Diabetes. 1999 May;48(5):975-82. [Abstract] [Full Text]
  • *Nohara O, Kulka M, Déry RE, Wills FL, Hirji NS, Gilchrist M, Befus AD: Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide. J Immunol. 2001 Nov 15;167(10):5935-9. [Abstract] [Full Text]
  • *Mabarrack NH, Turner NL, Mayrhofer G: Recent thymic origin, differentiation, and turnover of regulatory T cells. J Leukoc Biol. 2008 Nov;84(5):1287-97. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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