categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to CD83

HB15e (IgG1)

Product No.	Form	Quantity	Price
11-677-C025	purified	0.025 mg	choose region
11-677-C100	purified	0.1 mg	choose region
1F-677-T025	FITC	25 tests	choose region
1F-677-T100	FITC	100 tests	choose region
1P-677-T025	PE	25 tests	choose region
1P-677-T100	PE	100 tests	choose region
1A-677-T025	APC	25 tests	choose region
1A-677-T100	APC	100 tests	choose region

- In stock! Ready for immediate despatch

- Manufacture on order

Background

CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.

Entrez Gene: 6p23

Specificity

The mouse monoclonal antibody HB15e recognizes CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.
HLDA IV.; WS Code T 85

Immunogen

Human CD83-transfected Cos cells

Species Reactivity: Human Non-Human Primates Negative Species:	Applications: Flow Cytometry Positive control:peripheral blood-derived dendritic cells Immunohistochemistry (frozen sections) Application note:acetone fixation Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Loré K, Sönnerborg A, Broström C, Goh LE, Perrin L, McDade H, Stellbrink HJ, Gazzard B, Weber R, Napolitano LA, van Kooyk Y, Andersson J: Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection. AIDS. 2002 Mar 29;16(5):683-92. [Abstract] Cao W, Lee SH, Lu J: CD83 is preformed inside monocytes, macrophages and dendritic cells, but it is only stably expressed on activated dendritic cells. Biochem J. 2005 Jan 1;385(Pt 1):85-93. [Abstract] [Full Text] Klein E, Koch S, Borm B, Neumann J, Herzog V, Koch N, Bieber T: CD83 localization in a recycling compartment of immature human monocyte-derived dendritic cells. Int Immunol. 2005 Apr;17(4):477-87. [Abstract] [Full Text] Lundell AC, Andersson K, Josefsson E, Steinkasserer A, Rudin A: Soluble CD14 and CD83 from human neonatal antigen-presenting cells are inducible by commensal bacteria and suppress allergen-induced human neonatal Th2 differentiation. Infect Immun. 2007 Aug;75(8):4097-104. [Abstract] [Full Text] Kummer M, Turza NM, Muhl-Zurbes P, Lechmann M, Boutell C, Coffin RS, Everett RD, Steinkasserer A, Prechtel AT: Herpes simplex virus type 1 induces CD83 degradation in mature dendritic cells with immediate-early kinetics via the cellular proteasome. J Virol. 2007 Jun;81(12):6326-38. [Abstract] [Full Text] Wang Y, Dennehy PH, Keyserling HL, Tang K, Gentsch JR, Glass RI, Jiang B: Rotavirus infection alters peripheral T-cell homeostasis in children with acute diarrhea. J Virol. 2007 Apr;81(8):3904-12. [Abstract] [Full Text] Gerlini G, Mariotti G, Chiarugi A, Di Gennaro P, Caporale R, Parenti A, Cavone L, Tun-Kyi A, Prignano F, Saccardi R, Borgognoni L, Pimpinelli N: Induction of CD83+CD14+ nondendritic antigen-presenting cells by exposure of monocytes to IFN-alpha. J Immunol. 2008 Sep 1;181(5):2999-3008. [Abstract] [Full Text] Holmstrøm K, Pedersen AW, Claesson MH, Zocca MB, Jensen SS: Identification of a microRNA signature in dendritic cell vaccines for cancer immunotherapy. Hum Immunol. 2010 Jan;71(1):67-73. [Abstract] And many other.

Species Reactivity:

Human
Non-Human Primates

Negative Species:

Applications:

Flow Cytometry
Positive control:peripheral blood-derived dendritic cells
Immunohistochemistry (frozen sections)
Application note:acetone fixation

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

*Loré K, Sönnerborg A, Broström C, Goh LE, Perrin L, McDade H, Stellbrink HJ, Gazzard B, Weber R, Napolitano LA, van Kooyk Y, Andersson J: Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection. AIDS. 2002 Mar 29;16(5):683-92. [Abstract]

*Cao W, Lee SH, Lu J: CD83 is preformed inside monocytes, macrophages and dendritic cells, but it is only stably expressed on activated dendritic cells. Biochem J. 2005 Jan 1;385(Pt 1):85-93. [Abstract] [Full Text]

*Klein E, Koch S, Borm B, Neumann J, Herzog V, Koch N, Bieber T: CD83 localization in a recycling compartment of immature human monocyte-derived dendritic cells. Int Immunol. 2005 Apr;17(4):477-87. [Abstract] [Full Text]

*Lundell AC, Andersson K, Josefsson E, Steinkasserer A, Rudin A: Soluble CD14 and CD83 from human neonatal antigen-presenting cells are inducible by commensal bacteria and suppress allergen-induced human neonatal Th2 differentiation. Infect Immun. 2007 Aug;75(8):4097-104. [Abstract] [Full Text]

*Kummer M, Turza NM, Muhl-Zurbes P, Lechmann M, Boutell C, Coffin RS, Everett RD, Steinkasserer A, Prechtel AT: Herpes simplex virus type 1 induces CD83 degradation in mature dendritic cells with immediate-early kinetics via the cellular proteasome. J Virol. 2007 Jun;81(12):6326-38. [Abstract] [Full Text]

*Wang Y, Dennehy PH, Keyserling HL, Tang K, Gentsch JR, Glass RI, Jiang B: Rotavirus infection alters peripheral T-cell homeostasis in children with acute diarrhea. J Virol. 2007 Apr;81(8):3904-12. [Abstract] [Full Text]

*Gerlini G, Mariotti G, Chiarugi A, Di Gennaro P, Caporale R, Parenti A, Cavone L, Tun-Kyi A, Prignano F, Saccardi R, Borgognoni L, Pimpinelli N: Induction of CD83+CD14+ nondendritic antigen-presenting cells by exposure of monocytes to IFN-alpha. J Immunol. 2008 Sep 1;181(5):2999-3008. [Abstract] [Full Text]

*Holmstrøm K, Pedersen AW, Claesson MH, Zocca MB, Jensen SS: Identification of a microRNA signature in dendritic cell vaccines for cancer immunotherapy. Hum Immunol. 2010 Jan;71(1):67-73. [Abstract]

*And many other.

For research use only. Not for drug, diagnostic or other use.

PRODUCTS