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Mouse Monoclonal to CD83

HB15e (IgG1)

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  11-677-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-677-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-677-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-677-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-677-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-677-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-677-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-677-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  T8-677-T025 PE-Cy™5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T8-677-T100 PE-Cy™5 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.

Specificity

The mouse monoclonal antibody HB15e recognizes CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.
HLDA IV.; WS Code T 85

Regulatory Status

Immunogen

Human CD83-transfected Cos cells

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
    Positive control:peripheral blood-derived dendritic cells
  • Immunohistochemistry (frozen sections)
    Application note:acetone fixation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  • *Loré K, Sönnerborg A, Broström C, Goh LE, Perrin L, McDade H, Stellbrink HJ, Gazzard B, Weber R, Napolitano LA, van Kooyk Y, Andersson J: Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection. AIDS. 2002 Mar 29;16(5):683-92. [Abstract]
  • *Cao W, Lee SH, Lu J: CD83 is preformed inside monocytes, macrophages and dendritic cells, but it is only stably expressed on activated dendritic cells. Biochem J. 2005 Jan 1;385(Pt 1):85-93. [Abstract] [Full Text]
  • *Klein E, Koch S, Borm B, Neumann J, Herzog V, Koch N, Bieber T: CD83 localization in a recycling compartment of immature human monocyte-derived dendritic cells. Int Immunol. 2005 Apr;17(4):477-87. [Abstract] [Full Text]
  • *Lundell AC, Andersson K, Josefsson E, Steinkasserer A, Rudin A: Soluble CD14 and CD83 from human neonatal antigen-presenting cells are inducible by commensal bacteria and suppress allergen-induced human neonatal Th2 differentiation. Infect Immun. 2007 Aug;75(8):4097-104. [Abstract] [Full Text]
  • *Kummer M, Turza NM, Muhl-Zurbes P, Lechmann M, Boutell C, Coffin RS, Everett RD, Steinkasserer A, Prechtel AT: Herpes simplex virus type 1 induces CD83 degradation in mature dendritic cells with immediate-early kinetics via the cellular proteasome. J Virol. 2007 Jun;81(12):6326-38. [Abstract] [Full Text]
  • *Wang Y, Dennehy PH, Keyserling HL, Tang K, Gentsch JR, Glass RI, Jiang B: Rotavirus infection alters peripheral T-cell homeostasis in children with acute diarrhea. J Virol. 2007 Apr;81(8):3904-12. [Abstract] [Full Text]
  • *Gerlini G, Mariotti G, Chiarugi A, Di Gennaro P, Caporale R, Parenti A, Cavone L, Tun-Kyi A, Prignano F, Saccardi R, Borgognoni L, Pimpinelli N: Induction of CD83+CD14+ nondendritic antigen-presenting cells by exposure of monocytes to IFN-alpha. J Immunol. 2008 Sep 1;181(5):2999-3008. [Abstract] [Full Text]
  • *Holmstrøm K, Pedersen AW, Claesson MH, Zocca MB, Jensen SS: Identification of a microRNA signature in dendritic cell vaccines for cancer immunotherapy. Hum Immunol. 2010 Jan;71(1):67-73. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    HB15e FITC


    Fig. 1. Surface staining of CD83 in IFN alpha-activated human peripheral blood cells with anti-CD83 (HB15e) FITC.


    HB15e purified


    Fig. 2. Surface staining of CD83 in IFN alpha-activated human monocytes with anti-CD83 (HB15e) purified / GAM-APC.


    HB15e PE


    Fig. 3. Surface staining of CD83 in IFN alpha-activated human peripheral blood cells with anti-CD83 (HB15e) PE.

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