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Mouse Monoclonal to CD19

4G7 (IgG1)

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  11-663-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-663-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-663-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-663-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-663-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
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  PO-663-T025 Pacific Orange™ 25 tests yes choose region PDF datasheetHTML datasheet buy
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  1P-663-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
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  T5-663-T025 PE-DyLight® 594 25 tests yes choose region PDF datasheetHTML datasheet buy
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  1A-663-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
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  T8-663-T025 PE-Cy™5 25 tests yes choose region PDF datasheetHTML datasheet buy
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  PC-663-T025 PerCP 25 tests yes choose region PDF datasheetHTML datasheet buy
  PC-663-T100 PerCP 100 tests yes choose region PDF datasheetHTML datasheet buy
  T9-663-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T9-663-T100 PerCP-Cy™5.5 100 tests yes choose region PDF datasheetHTML datasheet buy
  A7-663-T025 Alexa Fluor® 700 25 tests yes choose region PDF datasheetHTML datasheet buy
  A7-663-T100 Alexa Fluor® 700 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

Specificity

The mouse monoclonal antibody 4G7 recognizes CD19 (B4), a 95 kDa type I transmembrane glycoprotein of immunoglobulin superfamily, expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
WS Code: 2 B43

Regulatory Status

Immunogen

Human CCL (chronic lymphocytic leukemia) cells

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1 μg/ml
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Leukocyte Typing II., Reinherz E.L. et al. (Eds.), Springer-Verlag (1985).
  • *Muench MO, Roncarolo MG, Namikawa R: Phenotypic and functional evidence for the expression of CD4 by hematopoietic stem cells isolated from human fetal liver. Blood. 1997 Feb 15;89(4):1364-75. [Abstract] [Full Text]
  • *Stockmeyer B, Dechant M, van Egmond M, Tutt AL, Sundarapandiyan K, Graziano RF, Repp R, Kalden JR, Gramatzki M, Glennie MJ, van de Winkel JG, Valerius T: Triggering Fc alpha-receptor I (CD89) recruits neutrophils as effector cells for CD20-directed antibody therapy. J Immunol. 2000 Nov 15;165(10):5954-61. [Abstract] [Full Text]
  • *Dubois B, Massacrier C, Caux C: Selective attraction of naive and memory B cells by dendritic cells. J Leukoc Biol. 2001 Oct;70(4):633-41. [Abstract] [Full Text]
  • *Basu S, Lynne CM, Ruiz P, Aballa TC, Ferrell SM, Brackett NL: Cytofluorographic identification of activated T-cell subpopulations in the semen of men with spinal cord injuries. J Androl. 2002 Jul-Aug;23(4):551-6. [Abstract] [Full Text]
  • *Köller M, Zwölfer B, Steiner G, Smolen JS, Scheinecker C: Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients. Int Immunol. 2004 Nov;16(11):1595-604. [Abstract] [Full Text]
  • *Treusch M, Vonthein R, Baur M, Günaydin I, Koch S, Stübiger N, Eckstein AK, Peter HH, Ness T, Zierhut M, Kötter I: Influence of human recombinant interferon-alpha2a (rhIFN-alpha2a) on altered lymphocyte subpopulations and monocytes in Behcet's disease. Rheumatology (Oxford). 2004 Oct;43(10):1275-82. [Abstract] [Full Text]
  • *Porcellini S, Vallanti G, Nozza S, Poli G, Lazzarin A, Tambussi G, Siccardi AG, Grassi F: Improved thymopoietic potential in aviremic HIV infected individuals treated with HAART by intermittent IL-2 administration. AIDS. 2003 Jul 25;17(11):1621-30. [Abstract]
  • *Andersen P, Pedersen MW, Woetmann A, Villingshøj M, Stockhausen MT, Odum N, Poulsen HS: EGFR induces expression of IRF-1 via STAT1 and STAT3 activation leading to growth arrest of human cancer cells. Int J Cancer. 2008 Jan 15;122(2):342-9. [Abstract] [Full Text]
  • *Martino V, Tonelli R, Montemurro L, Franzoni M, Marino F, Fazzina R, Pession A: Down-regulation of MLL-AF9, MLL and MYC expression is not obligatory for monocyte-macrophage maturation in AML-M5 cell lines carrying t(9;11)(p22;q23). Oncol Rep. 2006 Jan;15(1):207-11. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    4G7 pur


    Fig. 1. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) purified.


    4G7 PE-Cy7


    Fig. 2. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) Pacific OrangeTM.


    4G7 AF700


    Fig. 3. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) Alexa Fluor® 700.


    4G7 Pacific Orange


    Fig. 4. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) PE-CyTM7.



    4G7 APC FC


    Fig. 5. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) APC.


    4G7 PerCP


    Fig. 6. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) PerCP.


    4G7 PE-DyLight 594


    Fig. 7. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) PE-DyLight® 594.


    4G7 PE


    Fig. 8. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) PE.


    4G7 PC5


    Fig. 9. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) PE-CyTM5.



    4G7 PC55


    Fig. 10. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) PerCP-CyTM5.5.



    1B-663


    Fig. 11. Surface staining of human peripheral blood leukocytes with anti-human CD19 (4G7) biotin.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
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