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Mouse Monoclonal to CD16 / FcgammaRIII

3G8 (IgG1)

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  11-646-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  A4-646-T025 Alexa Fluor® 488 25 tests yes choose region PDF datasheetHTML datasheet buy
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  A6-646-T025 Alexa Fluor® 647 25 tests yes choose region PDF datasheetHTML datasheet buy
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  PC-646-T025 PerCP 25 tests yes choose region PDF datasheetHTML datasheet buy
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  T9-646-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Specificity

The mouse monoclonal antibody 3G8 recognizes CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).

HLDA V; WS Code NK80

Regulatory Status

Immunogen

Human neutrophils

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:6 μg/ml
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
    Application note:acetone fixation
  • Functional Application
    In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  • *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • *Zhu X, Hamann KJ, Muñoz NM, Rubio N, Mayer D, Herrnreiter A, Leff AR: Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils. J Immunol. 1998 Sep 1;161(5):2574-9. [Abstract] [Full Text]
  • *Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA: Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood. 1998 Apr 1;91(7):2369-80. [Abstract] [Full Text]
  • *Wijngaarden S, van Roon JA, van de Winkel JG, Bijlsma JW, Lafeber FP: Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment. [Abstract] [Full Text]
  • *Komano Y, Nanki T, Hayashida K, Taniguchi K, Miyasaka N: Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts. Arthritis Res Ther. 2006;8(5):R152. [Abstract] [Full Text]
  • *Choi EI, Wang R, Peterson L, Letvin NL, Reimann KA: Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques. Immunology. 2008 Jun;124(2):215-22. Epub 2008 Jan 12. [Abstract] [Full Text]
  • *Congy-Jolivet N, Bolzec A, Ternant D, Ohresser M, Watier H, Thibault G: Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype. Cancer Res. 2008 Feb 15;68(4):976-80. [Abstract] [Full Text]
  • *Burt BM, Plitas G, Zhao Z, Bamboat ZM, Nguyen HM, Dupont B, DeMatteo RP: The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors. J Immunol. 2009 Aug 1;183(3):1789-96. [Abstract] [Full Text]
  • *Jeraiby M, Sidi Yahya K, Depince-Berger AE, Lambert C: Microbicidal activity measured by flow cytometry: Optimization and standardization for detection of primary and functional deficiencies. J Immunol Methods. 2016 Sep 29. pii: S0022-1759(16)30220-4. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    3G8 APC-Cy7

    Fig. 1. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC-CyTM7.


    3G8 PE

    Fig. 2. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE.


    3G8 PE-Cy7

    Fig. 3. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE-CyTM7.


    3G8 PerCP FC

    Fig. 4. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerCP.


    3G8 PerCP-Cy5-5

    Fig. 5. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerC-CyTM5.5.


    3g8 PB

    Fig. 6. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific BlueTM.


    3G8 purified

    Fig. 7. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) purified / GAM-APC.



    3g8 af647

    Fig. 8. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 647.




    3G8 Pacific Orange

    Fig. 9. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific OrangeTM.


    1B-646

    Fig. 10. Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) biotin.


    1F-646

    Fig. 11. Surface staining of human peripheral blood with anti-CD16 (3G8) FITC.

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