categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to CD16 / FcgammaRIII

3G8 (IgG1)

Product No.	Form	Quantity	Price
11-646-C025	purified	0.025 mg	choose region
11-646-C100	purified	0.1 mg	choose region
12-646-C100	low endotoxin	0.1 mg	choose region
1B-646-C025	biotin	0.025 mg	choose region
1B-646-C100	biotin	0.1 mg	choose region
PB-646-T025	Pacific Blue™	25 tests	choose region
PB-646-T100	Pacific Blue™	100 tests	choose region
1F-646-T025	FITC	25 tests	choose region
1F-646-T100	FITC	100 tests	choose region
A4-646-T025	Alexa Fluor® 488	25 tests	choose region
A4-646-T100	Alexa Fluor® 488	100 tests	choose region
PO-646-T025	Pacific Orange™	25 tests	choose region
PO-646-T100	Pacific Orange™	100 tests	choose region
1P-646-T025	PE	25 tests	choose region
1P-646-T100	PE	100 tests	choose region
1A-646-T025	APC	25 tests	choose region
1A-646-T100	APC	100 tests	choose region
A6-646-T025	Alexa Fluor® 647	25 tests	choose region
A6-646-T100	Alexa Fluor® 647	100 tests	choose region
PC-646-T025	PerCP	25 tests	choose region
PC-646-T100	PerCP	100 tests	choose region
T9-646-T025	PerCP-Cy™5.5	25 tests	choose region
T9-646-T100	PerCP-Cy™5.5	100 tests	choose region
A7-646-T025	Alexa Fluor® 700	25 tests	choose region
A7-646-T100	Alexa Fluor® 700	100 tests	choose region

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Background

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Entrez Gene (human): 1q23

Specificity

The mouse monoclonal antibody 3G8 recognizes CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).

HLDA V; WS Code NK80

Immunogen

Human neutrophils

Species Reactivity: Human Non-Human Primates Negative Species:	Applications: Flow Cytometry Recommended dilution:6 μg/ml Immunoprecipitation Immunohistochemistry (frozen sections) Application note:acetone fixation Functional Application In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Zhu X, Hamann KJ, Muñoz NM, Rubio N, Mayer D, Herrnreiter A, Leff AR: Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils. J Immunol. 1998 Sep 1;161(5):2574-9. [Abstract] [Full Text] Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA: Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood. 1998 Apr 1;91(7):2369-80. [Abstract] [Full Text] Wijngaarden S, van Roon JA, van de Winkel JG, Bijlsma JW, Lafeber FP: Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment. [Abstract] [Full Text] Komano Y, Nanki T, Hayashida K, Taniguchi K, Miyasaka N: Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts. Arthritis Res Ther. 2006;8(5):R152. [Abstract] [Full Text] Choi EI, Wang R, Peterson L, Letvin NL, Reimann KA: Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques. Immunology. 2008 Jun;124(2):215-22. Epub 2008 Jan 12. [Abstract] [Full Text] Congy-Jolivet N, Bolzec A, Ternant D, Ohresser M, Watier H, Thibault G: Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype. Cancer Res. 2008 Feb 15;68(4):976-80. [Abstract] [Full Text] Burt BM, Plitas G, Zhao Z, Bamboat ZM, Nguyen HM, Dupont B, DeMatteo RP: The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors. J Immunol. 2009 Aug 1;183(3):1789-96. [Abstract] [Full Text] And many other.

Species Reactivity:

Human
Non-Human Primates

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:6 μg/ml
Immunoprecipitation
Immunohistochemistry (frozen sections)
Application note:acetone fixation
Functional Application
In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

*Zhu X, Hamann KJ, Muñoz NM, Rubio N, Mayer D, Herrnreiter A, Leff AR: Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils. J Immunol. 1998 Sep 1;161(5):2574-9. [Abstract] [Full Text]

*Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA: Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood. 1998 Apr 1;91(7):2369-80. [Abstract] [Full Text]

*Wijngaarden S, van Roon JA, van de Winkel JG, Bijlsma JW, Lafeber FP: Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment. [Abstract] [Full Text]

*Komano Y, Nanki T, Hayashida K, Taniguchi K, Miyasaka N: Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts. Arthritis Res Ther. 2006;8(5):R152. [Abstract] [Full Text]

*Choi EI, Wang R, Peterson L, Letvin NL, Reimann KA: Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques. Immunology. 2008 Jun;124(2):215-22. Epub 2008 Jan 12. [Abstract] [Full Text]

*Congy-Jolivet N, Bolzec A, Ternant D, Ohresser M, Watier H, Thibault G: Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype. Cancer Res. 2008 Feb 15;68(4):976-80. [Abstract] [Full Text]

*Burt BM, Plitas G, Zhao Z, Bamboat ZM, Nguyen HM, Dupont B, DeMatteo RP: The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors. J Immunol. 2009 Aug 1;183(3):1789-96. [Abstract] [Full Text]

*And many other.

For research use only. Not for drug, diagnostic or other use.

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