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Rat Monoclonal to CD25 (mouse) / IL-2R alpha chain

PC61.5 (IgG1)

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Background

CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.

Specificity

The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

Regulatory Status

Immunogen

B6.1 CTL cell line

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Functional Application
    In vitro blocking of IL-2 binding to its receptor (CD25) and inhibition of IL-2 stimulated cell growth. In vivo depletion of CD25 positive cells.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J Virol. 2000 Mar;74(5):2414-9. [Abstract] [Full Text]
  • *Kotner J, Tarleton R: Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007 Feb;75(2):861-9. [Abstract] [Full Text]
  • *Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J Immunol. 2008 Oct 15;181(8):5204-8. [Abstract] [Full Text]
  • *Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL-2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep;78(3):725-35. [Abstract] [Full Text]
  • *Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997 Jun 1;89(11):4005-12. [Abstract] [Full Text]
  • *Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection. Mol Cell Biol. 2002 Jul;22(13):4556-66. [Abstract] [Full Text]
  • *Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice. J Immunol. 2008 Nov 15;181(10):7221-9. [Abstract] [Full Text]
  • *Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J Biomed Biotechnol. 2010;2010:786078. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    PC61-5 FITC


    Fig. 1. Surface staining of CD25 in PHA activated (3 days) murine splenocytes using anti-CD25 (PC61.5) FITC.


    PC61 APC


    Fig. 2. Surface staining of CD25 in PHA activated (3 days) murine splenocytes using anti-CD25 (PC61.5) APC.

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