categories: CD and Related Antigens & MHC (Rodent)

Rat Monoclonal to CD25 (mouse) / IL-2R alpha chain

PC61.5 (IgG1)

Product No.	Form	Quantity	Price
11-614-C100	purified	0.1 mg	choose region
12-614-C100	low endotoxin	0.1 mg	choose region
1F-614-C100	FITC	0.1 mg	choose region
1P-614-C100	PE	0.1 mg	choose region

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- Manufacture on order

Background

CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.

Entrez Gene: 2 6.4 cM

Specificity

The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4⁺ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

Immunogen

B6.1 CTL cell line

Species Reactivity: Mouse Negative Species:	Applications: Flow Cytometry Immunoprecipitation Immunohistochemistry (frozen sections) Functional Application In vitro blocking of IL-2 binding to its receptor (CD25) and inhibition of IL-2 stimulated cell growth. In vivo depletion of CD25 positive cells. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J Virol. 2000 Mar;74(5):2414-9. [Abstract] [Full Text] Kotner J, Tarleton R: Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007 Feb;75(2):861-9. [Abstract] [Full Text] Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J Immunol. 2008 Oct 15;181(8):5204-8. [Abstract] [Full Text] Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL-2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep;78(3):725-35. [Abstract] [Full Text] Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997 Jun 1;89(11):4005-12. [Abstract] [Full Text] Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection. Mol Cell Biol. 2002 Jul;22(13):4556-66. [Abstract] [Full Text] Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice. J Immunol. 2008 Nov 15;181(10):7221-9. [Abstract] [Full Text] Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J Biomed Biotechnol. 2010;2010:786078. [Abstract] [Full Text] *And many other.

Species Reactivity:

Mouse

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Immunohistochemistry (frozen sections)
Functional Application
In vitro blocking of IL-2 binding to its receptor (CD25) and inhibition of IL-2 stimulated cell growth. In vivo depletion of CD25 positive cells.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J Virol. 2000 Mar;74(5):2414-9. [Abstract] [Full Text]

*Kotner J, Tarleton R: Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007 Feb;75(2):861-9. [Abstract] [Full Text]

*Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J Immunol. 2008 Oct 15;181(8):5204-8. [Abstract] [Full Text]

*Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL-2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep;78(3):725-35. [Abstract] [Full Text]

*Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997 Jun 1;89(11):4005-12. [Abstract] [Full Text]

*Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection. Mol Cell Biol. 2002 Jul;22(13):4556-66. [Abstract] [Full Text]

*Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice. J Immunol. 2008 Nov 15;181(10):7221-9. [Abstract] [Full Text]

*Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J Biomed Biotechnol. 2010;2010:786078. [Abstract] [Full Text]

*And many other.

For research use only. Not for drug, diagnostic or other use.

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