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Rabbit Polyclonal to PDK1 / PDPK1

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  11-659-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

PDK1 (3-phosphoinositide-dependent kinase 1), also known as PDPK1, is a key mediator of biological responses downstream of insulin and other tyrosine kinase receptors, regulating cell survival, cell cycle control, protein translation, and glucose metabolism. PDK1, containing a C-terminal pleckstrin homology domain, is activated in a phosphatidyl inositol 3,4,5-trisphosphate-dependent manner and phosphorylates multiple signaling molecules, including PKB/Akt, PKC, p70S6 kinase, serum and glucocorticoid-induced kinase. In T cells, nucleation of TCR-induced signaling complex for NFkappaB activation pathway is another crucial role of PDK1. Subcellular localization of PDK1 depends on conditions and has not been completely understood.

Specificity

The rabbit polyclonal antibody recognizes PDK1, a serine/threonine proteinkinase of 63 kDa, which is a central activator of multiple signaling pathways coupled to a large number of growth-promoting stimuli.

Regulatory Status

Immunogen

Peptide corresponding to the amino acids CIQEVWRQRYQSHPDAAVQ of human PDK1

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution:2 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Scheid MP, Parsons M, Woodgett JR: Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation. Mol Cell Biol. 2005 Mar;25(6):2347-63. [Abstract] [Full Text]
  • *Egawa K, Maegawa H, Shi K, Nakamura T, Obata T, Yoshizaki T, Morino K, Shimizu S, Nishio Y, Suzuki E, Kashiwagi A: Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in 3T3-L1 adipocytes. J Biol Chem. 2002 Oct 11;277(41):38863-9. [Abstract] [Full Text]
  • *Lee KY, D'Acquisto F, Hayden MS, Shim JH, Ghosh S: PDK1 nucleates T cell receptor-induced signaling complex for NF-kappaB activation. Science. 2005 Apr 1;308(5718):114-8. [Abstract]
  • *Alajajian BB, Fletcher L, Isgor E, Jimenez DF, Digicaylioglu M: IGF-I regulated phosphorylation and translocation of PDK-1 in neurons. Neuroreport. 2009 Apr 22;20(6):579-83. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

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