Rabbit Polyclonal to MRCK alpha
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(myotonic dystrophy kinase-related Cdc42-binding kinase alpha) is a member of the dystrophia myotonica protein kinase (DMPK) family that functions downstream of Cdc42 in actin cytoskeletal reorganization. It is a serine/threonine kinase with multiple functional domains, including phorbol ester-responsive C1 domain. Three independent coiled-coil domains and the N-terminal region preceding the kinase domain are responsible for intermolecular interactions leading to MRCK alpha multimerization. The transautophosphorylation process is critical for regulation of MRCK alpha catalytic activities. Binding of phorbol esters to MRCK releases its autoinhibition, allowing N-terminal dimerization and subsequent kinase activation.
The polyclonal antibody anti-MRCK alpha recognizes myotonic dystrophy kinase-related Cdc42-binding kinase alpha, a 180 kDa Ser/Thr kinase, which is responsible for reorganization of actin cytoskeleton.
Peptide corresponding to the amino acids 1671-1685 of human MRCK alpha
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Choi SH, Czifra G, Kedei N, Lewin NE, Lazar J, Pu Y, Marquez VE, Blumberg PM: Characterization of the interaction of phorbol esters with the C1 domain of MRCK (myotonic dystrophy kinase-related Cdc42 binding kinase) alpha/beta. J Biol Chem. 2008 Apr 18;283(16):10543-9. [Abstract] [Full Text]
*Tan I, Ng CH, Lim L, Leung T: Phosphorylation of a novel myosin binding subunit of protein phosphatase 1 reveals a conserved mechanism in the regulation of actin cytoskeleton. J Biol Chem. 2001 Jun 15;276(24):21209-16. [Abstract] [Full Text]
*Tan I, Seow KT, Lim L, Leung T: Intermolecular and intramolecular interactions regulate catalytic activity of
myotonic dystrophy kinase-related Cdc42-binding kinase alpha. Mol Cell Biol. 2001 Apr;21(8):2767-78. [Abstract] [Full Text]
Product Specific References
*Cmejla R, Ptackova P, Petrak J, Savvulidi F, Cerny J, Sebesta O, Vyoral D: Human MRCKalpha is regulated by cellular iron levels and interferes with transferrin iron uptake. Biochem Biophys Res Commun. 2010 Apr 30;395(2):163-7. [Abstract]
For research use only. Not for drug, diagnostic or other use.
Colocalization between endogenous MRCK (red) and transferrin-Dyomics 547 (green) after 10 min. incubation of the cells (HeLa) with transferrin-Dyomics 547.
Immunostaining with polyclonal anti-MRCK alpha
Colocalization between endogenous MRCK (red) and transfected MRCK-EGFP (green) HeLa cells. Immunostaining with polyclonal anti-MRCK alpha