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Mouse IgG1 Isotype Control

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Product No. Form Quantity Stock Price Datasheet Shop
  11-632-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  PC-632-C025 PerCP 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  PC-632-C100 PerCP 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  T9-632-C025 PerCP-Cy™5.5 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  A7-632-C025 Alexa Fluor® 700 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Specificity

This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.

Regulatory Status

Negative Species:

  • Human
  • Rat

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Control Experiments
    The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25. [Abstract] [Full Text]
  • *Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46. [Abstract] [Full Text]
  • *Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99. [Abstract] [Full Text]
  • *Wiendl H, Mitsdoerffer M, Schneider D, Melms A, Lochmuller H, Hohlfeld R, Weller M: Muscle fibres and cultured muscle cells express the B7.1/2-related inducible co-stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies. Brain. 2003 May;126(Pt 5):1026-35. [Abstract] [Full Text]
  • *Bryceson YT, March ME, Barber DF, Ljunggren HG, Long EO: Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells. J Exp Med. 2005 Oct 3;202(7):1001-12. [Abstract] [Full Text]
  • *Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

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  • Example Data

    MOPC-21 PerCP-Cy5-5


    Fig. 1. Example of nonspecific mouse IgG1 (MOPC-21) PerCP-CyTM5.5 signal on human peripheral blood; surface staining, 27 μg/ml.


    MOPC-21 Pacific Orange


    Fig. 2. Example of nonspecific mouse IgG1 (MOPC-21) Pacific OrangeTM signal on human peripheral blood; surface staining, 3 μg/ml.


    MOPC-21 PAC BL


    Fig. 3. Example of nonspecific mouse IgG1 (MOPC-21) Pacific BlueTM signal on human peripheral blood; surface staining, 3 μg/ml.



    MOPC-21 PE-DyLight 594


    Fig. 4. Example of nonspecific mouse IgG1 (MOPC-21) PE-DyLight® 594 signal on human peripheral blood; surface staining, 1 μg/ml.



    MOPC-21 APC-Cy7 FC


    Fig. 5. Example of nonspecific mouse IgG1 (MOPC-21) APC-CyTM7 signal on human peripheral blood; surface staining, 3 μg/ml.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
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