categories: Mouse Immunoglobulin Isotype Controls

Mouse IgG1 Isotype Control

Product No.	Form	Quantity	Price
11-632-C025	purified	0.025 mg	choose region
11-632-C100	purified	0.1 mg	choose region
12-632-C025	low endotoxin	0.025 mg	choose region
12-632-C100	low endotoxin	0.1 mg	choose region
1B-632-C025	biotin	0.025 mg	choose region
1B-632-C100	biotin	0.1 mg	choose region
PB-632-C025	Pacific Blue™	0.025 mg	choose region
PB-632-C100	Pacific Blue™	0.1 mg	choose region
1F-632-C025	FITC	0.025 mg	choose region
1F-632-C100	FITC	0.1 mg	choose region
A4-632-C025	Alexa Fluor® 488	0.025 mg	choose region
A4-632-C100	Alexa Fluor® 488	0.1 mg	choose region
PO-632-C025	Pacific Orange™	0.025 mg	choose region
PO-632-C100	Pacific Orange™	0.1 mg	choose region
1P-632-C025	PE	0.025 mg	choose region
1P-632-C100	PE	0.1 mg	choose region
1A-632-C025	APC	0.025 mg	choose region
1A-632-C100	APC	0.1 mg	choose region
T8-632-C025	PE-Cy™5	0.025 mg	choose region
T8-632-C100	PE-Cy™5	0.1 mg	choose region
A6-632-C025	Alexa Fluor® 647	0.025 mg	choose region
A6-632-C100	Alexa Fluor® 647	0.1 mg	choose region
PC-632-C025	PerCP	0.025 mg	choose region
PC-632-C100	PerCP	0.1 mg	choose region
T9-632-C025	PerCP-Cy™5.5	0.025 mg	choose region
T9-632-C100	PerCP-Cy™5.5	0.1 mg	choose region
A7-632-C025	Alexa Fluor® 700	0.025 mg	choose region
A7-632-C100	Alexa Fluor® 700	0.1 mg	choose region

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Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Specificity

This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.

Negative Species: Human Rat	Applications: Flow Cytometry Immunoprecipitation Western Blotting Immunohistochemistry (paraffin sections) Immunohistochemistry (frozen sections) Control Experiments The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25. [Abstract] [Full Text] Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46. [Abstract] [Full Text] Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99. [Abstract] [Full Text] Wiendl H, Mitsdoerffer M, Schneider D, Melms A, Lochmuller H, Hohlfeld R, Weller M: Muscle fibres and cultured muscle cells express the B7.1/2-related inducible co-stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies. Brain. 2003 May;126(Pt 5):1026-35. [Abstract] [Full Text] Bryceson YT, March ME, Barber DF, Ljunggren HG, Long EO: Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells. J Exp Med. 2005 Oct 3;202(7):1001-12. [Abstract] [Full Text] Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936. [Abstract] [Full Text]

Negative Species:

Human
Rat

Applications:

Flow Cytometry
Immunoprecipitation
Western Blotting
Immunohistochemistry (paraffin sections)
Immunohistochemistry (frozen sections)
Control Experiments
The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25. [Abstract] [Full Text]

*Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46. [Abstract] [Full Text]

*Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99. [Abstract] [Full Text]

*Wiendl H, Mitsdoerffer M, Schneider D, Melms A, Lochmuller H, Hohlfeld R, Weller M: Muscle fibres and cultured muscle cells express the B7.1/2-related inducible co-stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies. Brain. 2003 May;126(Pt 5):1026-35. [Abstract] [Full Text]

*Bryceson YT, March ME, Barber DF, Ljunggren HG, Long EO: Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells. J Exp Med. 2005 Oct 3;202(7):1001-12. [Abstract] [Full Text]

*Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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