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Mouse Monoclonal to CD62L / L-Selectin

DREG56 (IgG1)

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  11-630-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  12-630-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-630-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-630-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  T9-630-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T9-630-T100 PerCP-Cy™5.5 100 tests yes choose region PDF datasheetHTML datasheet buy
  A7-630-T025 Alexa Fluor® 700 25 tests yes choose region PDF datasheetHTML datasheet buy
  A7-630-T100 Alexa Fluor® 700 100 tests yes choose region PDF datasheetHTML datasheet buy
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CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.


The mouse monoclonal antibody DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.
HLDA V; WS Code S056

Regulatory Status


PMA-activated human peripheral blood leukocytes

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
    Recommended dilution:2 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Application note:non-reducing conditions
  • Immunohistochemistry (frozen sections)
  • Functional Application
    Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Kishimoto TK, Jutila MA, Butcher EC: Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc Natl Acad Sci U S A. 1990 Mar;87(6):2244-8. [Abstract] [Full Text]
  • *Kishimoto TK, Warnock RA, Jutila MA, Butcher EC, Lane C, Anderson DC, Smith CW: Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro. Blood. 1991 Aug 1;78(3):805-11. [Abstract] [Full Text]
  • *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM: L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts. J Immunol. 2002 Aug 15;169(4):1768-73. [Abstract] [Full Text]
  • *Abraham WM, Ahmed A, Sabater JR, Lauredo IT, Botvinnikova Y, Bjercke RJ, Hu X, Revelle BM, Kogan TP, Scott IL, Dixon RA, Yeh ET, Beck PJ: Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep. Am J Respir Crit Care Med. 1999 Apr;159(4 Pt 1):1205-14. [Abstract] [Full Text]
  • *Xu T, Chen L, Shang X, Cui L, Luo J, Chen C, Ba X, Zeng X: Critical role of Lck in L-selectin signaling induced by sulfatides engagement. J Leukoc Biol. 2008 Oct;84(4):1192-201. [Abstract] [Full Text]
  • *Killock DJ, Parsons M, Zarrouk M, Ameer-Beg SM, Ridley AJ, Haskard DO, Zvelebil M, Ivetic A: In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin. J Biol Chem. 2009 Mar 27;284(13):8833-45. [Abstract] [Full Text]
  • *Tu W, Mao H, Zheng J, Liu Y, Chiu SS, Qin G, Chan PL, Lam KT, Guan J, Zhang L, Guan Y, Yuen KY, Peiris JS, Lau YL: Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus. J Virol. 2010 Jul;84(13):6527-35. [Abstract] [Full Text]
  • *And other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    DREG56 AF700

    Fig. 1. Surface staining of CD62L in human peripheral blood with anti-CD62L (DREG56) Alexa Fluor 700.

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