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Mouse Monoclonal to PRR7 / TRAP3

TRAP3/10 (IgG2a)

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Background

PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.

Specificity

The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.

Regulatory Status

Immunogen

Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)

Species Reactivity:

  • Human
  • Mouse
  • Rat

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution:1 μg/ml
    Positive control:murine brain lysate (red. Laemmli buffer)
  • Immunocytochemistry
    Recommended dilution:10 μg/ml
    Application note:Cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Murata Y, Doi T, Taniguchi H, Fujiyoshi Y: Proteomic analysis revealed a novel synaptic proline-rich membrane protein (PRR7) associated with PSD-95 and NMDA receptor. Biochem Biophys Res Commun. 2005 Feb 4;327(1):183-91. [Abstract]
  • Product Specific References

  • *Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem. 2011 Jun 3;286(22):19617-29 [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    PRR7 ICC
    Fig. 1.


    Fig. 1. Immunofluorescence staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).


    prr wb
    Fig. 2.


    Fig. 2. Detection of PRR7 / TRAP3 in murine brain lysate by Western blotting using the monoclonal antibody TRAP3/10.

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