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Hamster Monoclonal to CD154 / CD40L (mouse)

MR-1 (IgG1)

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  11-629-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.

Specificity

The Armenian hamster monoclonal antibody MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

Regulatory Status

Immunogen

Activated mouse Th1 clone D1.6

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:4 μg/ml
    Positive control:6-8 hour activated mouse splenocytes
  • Immunoprecipitation
  • Immunohistochemistry
  • Immunocytochemistry
  • Functional Application
    Blocking T cell costimulation in vivo.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A: A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6550-4. [Abstract] [Full Text]
  • *Howard LM, Miga AJ, Vanderlugt CL, Dal Canto MC, Laman JD, Noelle RJ, Miller SD: Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis. J Clin Invest. 1999 Jan;103(2):281-90. [Abstract] [Full Text]
  • *Shin T, Kennedy G, Gorski K, Tsuchiya H, Koseki H, Azuma M, Yagita H, Chen L, Powell J, Pardoll D, Housseau F: Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor. J Exp Med. 2003 Jul 7;198(1):31-8. [Abstract] [Full Text]
  • *Luzina IG, Atamas SP, Storrer CE, daSilva LC, Kelsoe G, Papadimitriou JC, Handwerger BS: Spontaneous formation of germinal centers in autoimmune mice. J Leukoc Biol. 2001 Oct;70(4):578-84. [Abstract] [Full Text]
  • *Wu Y, Zhou Q, Zheng P, Liu Y: CD28-independent induction of T helper cells and immunoglobulin class switches requires costimulation by the heat-stable antigen. J Exp Med. 1998 Apr 6;187(7):1151-6. [Abstract]
  • *Salgado CG, Nakamura K, Sugaya M, Tada Y, Asahina A, Koyama Y, Irie S, Tamaki K: Functional CD40 ligand is expressed on epidermal Langerhans cells. J Leukoc Biol. 1999 Aug;66(2):281-5. [Abstract] [Full Text]
  • *Murakawa T, Kerklo MM, Zamora MR, Wei Y, Gill RG, Henson PM, Grover FL, Nicolls MR: Simultaneous LFA-1 and CD40 ligand antagonism prevents airway remodeling in orthotopic airway transplantation: implications for the role of respiratory epithelium as a modulator of fibrosis. J Immunol. 2005 Apr 1;174(7):3869-79. [Abstract] [Full Text]
  • *Hwang Y, Nahm MH, Briles DE, Thomas D, Purkerson JM: Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L. Infect Immun. 2000 Feb;68(2):511-7. [Abstract] [Full Text]
  • *Fischbein MP, Ardehali A, Yun J, Schoenberger S, Laks H, Irie Y, Dempsey P, Cheng G, Fishbein MC, Bonavida B: CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants. J Immunol. 2000 Dec 15;165(12):7316-22. [Abstract] [Full Text]
  • *Lode HN, Xiang R, Pertl U, F÷rster E, Schoenberger SP, Gillies SD, Reisfeld RA: Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction. J Clin Invest. 2000 Jun;105(11):1623-30. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO┤s term and conditions which are available at www.exbio.cz.
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