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Rat Monoclonal to CD62L / L-Selectin (mouse)

MEL-14 (IgG2a)

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Background

CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).

Specificity

The rat monoclonal antibody MEL-14 reacts with mouse CD62L (L-selectin), a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.

Regulatory Status

Immunogen

C3H/eb mouse B cell lymphoma 38C-13

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 0.5 μg/ml
  • Immunoprecipitation
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • Functional Application
    Blocking of cell adhesion
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Gallatin WM, Weissman IL, Butcher EC: A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature 1983 Jul 7;304:30-34. [Abstract] [Full Text]
  • *Brawand P, Cerottini JC, MacDonald HR: Hierarchal utilization of different T-cell receptor Vbeta gene segments in the CD8(+)-T-cell response to an immunodominant Moloney leukemia virus-encoded epitope in vivo. J Virol. 1999 Nov;73(11):9161-9. [Abstract] [Full Text]
  • *Fisson S, Darrasse-Jèze G, Litvinova E, Septier F, Klatzmann D, Liblau R, Salomon BL: Continuous activation of autoreactive CD4+ CD25+ regulatory T cells in the steady state. J Exp Med. 2003 Sep 1;198(5):737-46. [Abstract] [Full Text]
  • *ten Bruggencate SJ, Hillyer LM, Woodward BD: The proportion of CD45RA(+)CD62L(+) (quiescent-phenotype) T cells within the CD8(+) subset increases in advanced weight loss in the protein- or energy-deficient weanling mouse. J Nutr. 2001 Dec;131(12):3266-9. [Abstract] [Full Text]
  • *Mannering SI, Cheers C: Interleukin-2 and loss of immunity in experimental Mycobacterium avium infection. Infect Immun. 2002 Jan;70(1):27-35. [Abstract] [Full Text]
  • *Pop SM, Wong CP, He Q, Wang Y, Wallet MA, Goudy KS, Tisch R: The type and frequency of immunoregulatory CD4+ T-cells govern the efficacy of antigen-specific immunotherapy in nonobese diabetic mice. Diabetes. 2007 May;56(5):1395-402. [Abstract] [Full Text]
  • *Chin CS, Miller CH, Graham L, Parviz M, Zacur S, Patel B, Duong A, Bear HD: Bryostatin 1/ionomycin (B/I) ex vivo stimulation preferentially activates L-selectinlow tumor-sensitized lymphocytes. Int Immunol. 2004 Sep;16(9):1283-94. [Abstract] [Full Text]
  • *Chen BJ, Cui X, Sempowski GD, Liu C, Chao NJ: Transfer of allogeneic CD62L- memory T cells without graft-versus-host disease. Blood. 2004 Feb 15;103(4):1534-41. [Abstract] [Full Text]
  • *Xu H, Manivannan A, Crane I, Dawson R, Liversidge J: Critical but divergent roles for CD62L and CD44 in directing blood monocyte trafficking in vivo during inflammation. Blood. 2008 Aug 15;112(4):1166-74. [Abstract] [Full Text]
  • *Friedline RH, Wong CP, Steeber DA, Tedder TF, Tisch R: L-selectin is not required for T cell-mediated autoimmune diabetes. J Immunol. 2002 Mar 15;168(6):2659-66. [Abstract] [Full Text]
  • *Richards H, Longhi MP, Wright K, Gallimore A, Ager A: CD62L (L-selectin) down-regulation does not affect memory T cell distribution but failure to shed compromises anti-viral immunity. J Immunol. 2008 Jan 1;180(1):198-206. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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