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Rat Monoclonal to CD44

IM7 (IgG2b)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-624-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-624-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-624-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-624-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-624-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  T9-624-C025 PerCP-Cy™5.5 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  T9-624-C100 PerCP-Cy™5.5 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  T7-624-C025 PE-Cy™7 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  T7-624-C100 PE-Cy™7 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Specificity

The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.

Regulatory Status

Immunogen

Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1

Species Reactivity:

  • Human
  • Mouse
  • Canine (Dog)
  • Equine (Horse)
  • Feline (Cat)

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 1 μg/ml
    Positive control:mouse peripheral blood
  • Immunoprecipitation
  • Western Blotting
    Application note: non-reducing conditions
  • Immunohistochemistry (paraffin sections)
    Positive tissue:mouse spleen
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Lesley J, Trowbridge IS: Genetic characterization of a polymorphic murine cell-surface glycoprotein. Immunogenetics. 1982 Mar;15(3):313-20. [Abstract]
  • *Hegde VL, Singh NP, Nagarkatti PS, Nagarkatti M: CD44 mobilization in allogeneic dendritic cell-T cell immunological synapse plays a key role in T cell activation. J Leukoc Biol. 2008 Jul;84(1):134-42. [Abstract] [Full Text]
  • *van Royen N, Voskuil M, Hoefer I, Jost M, de Graaf S, Hedwig F, Andert JP, Wormhoudt TA, Hua J, Hartmann S, Bode C, Buschmann I, Schaper W, van der Neut R, Piek JJ, Pals ST: CD44 regulates arteriogenesis in mice and is differentially expressed in patients with poor and good collateralization. Circulation. 2004 Apr 6;109(13):1647-52. [Abstract] [Full Text]
  • *McKallip RJ, Do Y, Fisher MT, Robertson JL, Nagarkatti PS, Nagarkatti M: Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens. Int Immunol. 2002 Sep;14(9):1015-26. [Abstract] [Full Text]
  • *Katoh S, Matsumoto N, Kawakita K, Tominaga A, Kincade PW, Matsukura S: A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia. J Clin Invest. 2003 May;111(10):1563-70. [Abstract] [Full Text]
  • *Legg JW, Lewis CA, Parsons M, Ng T, Isacke CM: A novel PKC-regulated mechanism controls CD44 ezrin association and directional cell motility. Nat Cell Biol. 2002 Jun;4(6):399-407. [Abstract] [Full Text]
  • *Si-Tahar M, Sitaraman S, Shibahara T, Madara JL: Negative regulation of epithelium-neutrophil interactions via activation of CD44. Am J Physiol Cell Physiol. 2001 Mar;280(3):C423-32. [Abstract] [Full Text]
  • *Larkin J, Renukaradhya GJ, Sriram V, Du W, Gervay-Hague J, Brutkiewicz RR: CD44 differentially activates mouse NK T cells and conventional T cells. J Immunol. 2006 Jul 1;177(1):268-79. [Abstract] [Full Text]
  • *Xu H, Manivannan A, Liversidge J, Sharp PF, Forrester JV, Crane IJ: Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier. J Leukoc Biol. 2002 Dec;72(6):1133-41. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    IM7 purified WB


    Fig. 1. Western blotting analysis of CD44 in HeLa cells (positive; lane 2) and MOLT-4 cells (negative; lane 1) using anti-CD44 (IM7) purified; non-reducing conditions.


    IM7 APC


    Fig. 2. Surface staining of murine splenocytes with anti-CD44 (IM7) APC.


    IM7 FITC


    Fig. 3. Surface staining of murine splenocytes with anti-CD44 (IM7) FITC.


    IM7 purified


    Fig. 4. Surface staining of murine splenocytes with anti-CD44 (IM7) purified / DAR-APC.


    IM7 PC7


    Fig. 5. Surface staining of murine splenocytes with anti-CD44 (IM7) PE-CyTM7.


    1B-624


    Fig. 6. Surface staining of murine splenocytes with anti-CD44 (IM7) biotin.


    1P-624


    Fig. 7. Surface staining of murine splenocytes with anti-CD44 (IM7) PE.



    IM7 PerCP-Cy5.5


    Fig. 8. Surface staining of murine splenocytes with anti-CD44 (IM7) PerCP-CyTM5.5.

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