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Rat Monoclonal to CD18 (mouse) / Integrin beta2 subunit

M18/2 (IgG2a)

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Background

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts.

Specificity

The rat monoclonal antibody M18/2 recognizes CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.

Regulatory Status

Immunogen

Murine cytotoxic T cell glycoproteins

Species Reactivity:

  • Mouse

Negative Species:

  • Canine (Dog)

Applications:

  • Flow Cytometry
    Recommended dilution: 1 μg/ml
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Functional Application
    Blocking or stimulation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983 Aug 1;158(2):586-602. [Abstract] [Full Text]
  • *Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18). J Immunol. 1993 May 15;150(10):4466-77. [Abstract]
  • *Zahalka MA, Naor D: Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness. Int Immunol. 1994 Jun;6(6):917-24. [Abstract]
  • *Watts GM, Beurskens FJ, Martin-Padura I, Ballantyne CM, Klickstein LB, Brenner MB, Lee DM: Manifestations of inflammatory arthritis are critically dependent on LFA-1. J Immunol. 2005 Mar 15;174(6):3668-75. [Abstract] [Full Text]
  • *Avni O, Pur Z, Yefenof E, Baniyash M: Complement receptor 3 of macrophages is associated with galectin-1-like protein. J Immunol. 1998 Jun 15;160(12):6151-8. [Abstract] [Full Text]
  • *Sakurai E, Taguchi H, Anand A, Ambati BK, Gragoudas ES, Miller JW, Adamis AP, Ambati J: Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003 Jun;44(6):2743-9. [Abstract] [Full Text]
  • *Cullere X, Lauterbach M, Tsuboi N, Mayadas TN: Neutrophil-selective CD18 silencing using RNA interference in vivo. Blood. 2008 Apr 1;111(7):3591-8. [Abstract] [Full Text]
  • *Barlow SC, Langston W, Matthews KM, Chidlow JH Jr, Kevil CG: CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes. Am J Pathol. 2004 Dec;165(6):1849-52. [Abstract] [Full Text]
  • *Varga G, Balkow S, Wild MK, Stadtbaeumer A, Krummen M, Rothoeft T, Higuchi T, Beissert S, Wethmar K, Scharffetter-Kochanek K, Vestweber D, Grabbe S: Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation. Blood. 2007 Jan 15;109(2):661-9. [Abstract] [Full Text]
  • *Abraham C, Miller J: Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1. J Immunol. 2001 Nov 1;167(9):5193-201. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    m18-2
    Fig. 1.

    Fig. 1. Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2).


    M18-2 FITC

    Fig. 2. Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2) FITC.

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