categories: CD and Related Antigens & MHC (Rodent)

Rat Monoclonal to CD11a (mouse) / LFA-1 alpha chain

M17/4 (IgG2a)

Product No.	Form	Quantity	Price
11-622-C100	purified	0.1 mg	choose region
12-622-C100	low endotoxin	0.1 mg	choose region
1F-622-C100	FITC	0.1 mg	choose region

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Background

CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions.

Entrez Gene: 7 60.0 cM

Specificity

The rat monoclonal antibody M17/4 reacts with CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

Immunogen

C57BL/6 mouse splenic secondary cytotoxic T lymphocytes

Species Reactivity: Mouse Negative Species:	Applications: Flow Cytometry Recommended dilution: 1 μg/ml Immunoprecipitation Immunohistochemistry (frozen sections) Positive tissue:mouse spleen or thymus Application note:acetone fixation Functional Application Blocking Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Sanchez-Madrid F, Davignon D, Martz E, Springer TA: Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell Immunol. 1982 Oct;73(1):1-11. [Abstract] Kuhlman P, Moy VT, Lollo BA, Brian AA: The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation. J Immunol. 1991 Mar 15;146(6):1773-82. [Abstract] Angelov GS, Guillaume P, Cebecauer M, Bosshard G, Dojcinovic D, Baumgaertner P, Luescher IF: Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity. J Immunol. 2006 Mar 15;176(6):3356-65. [Abstract] [Full Text] Smith SS, Barnum SR: Differential expression of beta 2-integrins and cytokine production between gammadelta and alphabeta T cells in experimental autoimmune encephalomyelitis. J Leukoc Biol. 2008 Jan;83(1):71-9. [Abstract] [Full Text] Yung R, Powers D, Johnson K, Amento E, Carr D, Laing T, Yang J, Chang S, Hemati N, Richardson B: Mechanisms of drug-induced lupus. II. T cells overexpressing lymphocyte function-associated antigen 1 become autoreactive and cause a lupuslike disease in syngeneic mice. J Clin Invest. 1996 Jun 15;97(12):2866-71. [Abstract] [Full Text] Bellingan GJ, Xu P, Cooksley H, Cauldwell H, Shock A, Bottoms S, Haslett C, Mutsaers SE, Laurent GJ: Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J Exp Med. 2002 Dec 2;196(11):1515-21. [Abstract] [Full Text] Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J: The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo. Respir Res. 2005 Jul 12;6:70. [Abstract] [Full Text] Koseki S, Miura S, Fujimori H, Hokari R, Komoto S, Hara Y, Ogino T, Nagata H, Goto M, Hachimura S, Kaminogawa S, Ishii H: In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine. Int Immunol. 2001 Sep;13(9):1165-74. [Abstract] [Full Text] Papayannopoulou T, Priestley GV, Nakamoto B, Zafiropoulos V, Scott LM, Harlan JM: Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice. Blood. 2001 Mar 1;97(5):1282-8. [Abstract] [Full Text] And many other.

Species Reactivity:

Mouse

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 1 μg/ml
Immunoprecipitation
Immunohistochemistry (frozen sections)
Positive tissue:mouse spleen or thymus
Application note:acetone fixation
Functional Application
Blocking

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Sanchez-Madrid F, Davignon D, Martz E, Springer TA: Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell Immunol. 1982 Oct;73(1):1-11. [Abstract]

*Kuhlman P, Moy VT, Lollo BA, Brian AA: The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation. J Immunol. 1991 Mar 15;146(6):1773-82. [Abstract]

*Angelov GS, Guillaume P, Cebecauer M, Bosshard G, Dojcinovic D, Baumgaertner P, Luescher IF: Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity. J Immunol. 2006 Mar 15;176(6):3356-65. [Abstract] [Full Text]

*Smith SS, Barnum SR: Differential expression of beta 2-integrins and cytokine production between gammadelta and alphabeta T cells in experimental autoimmune encephalomyelitis. J Leukoc Biol. 2008 Jan;83(1):71-9. [Abstract] [Full Text]

*Yung R, Powers D, Johnson K, Amento E, Carr D, Laing T, Yang J, Chang S, Hemati N, Richardson B: Mechanisms of drug-induced lupus. II. T cells overexpressing lymphocyte function-associated antigen 1 become autoreactive and cause a lupuslike disease in syngeneic mice. J Clin Invest. 1996 Jun 15;97(12):2866-71. [Abstract] [Full Text]

*Bellingan GJ, Xu P, Cooksley H, Cauldwell H, Shock A, Bottoms S, Haslett C, Mutsaers SE, Laurent GJ: Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J Exp Med. 2002 Dec 2;196(11):1515-21. [Abstract] [Full Text]

*Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J: The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo. Respir Res. 2005 Jul 12;6:70. [Abstract] [Full Text]

*Koseki S, Miura S, Fujimori H, Hokari R, Komoto S, Hara Y, Ogino T, Nagata H, Goto M, Hachimura S, Kaminogawa S, Ishii H: In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine. Int Immunol. 2001 Sep;13(9):1165-74. [Abstract] [Full Text]

*Papayannopoulou T, Priestley GV, Nakamoto B, Zafiropoulos V, Scott LM, Harlan JM: Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice. Blood. 2001 Mar 1;97(5):1282-8. [Abstract] [Full Text]

*And many other.

For research use only. Not for drug, diagnostic or other use.

Example Data

Fig. 1.

Fig. 1. Surface staining of mouse splenocytes using anti-CD11a monoclonal antibody (clone M17/4).

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