System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Rat Monoclonal to MHC Class II (mouse)

M5/114 (IgG2b)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  10-621-C100 azide free 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-621-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

Specificity

The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Regulatory Status

Immunogen

Activated C57BL/6 mouse spleen cells

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 2 μg/ml
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Functional Application
    Blocking of T cell proliferative responses
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Bhattacharya A, Dorf ME, Springer TA: A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol. 1981 Dec;127(6):2488-95. [Abstract]
  • *Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, Benoist C, Mathis D: Mice lacking the MHC class II-associated invariant chain. Cell. 1993 Feb 26;72(4):635-48. [Abstract]
  • *De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC: Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J Cell Sci. 1995 Oct;108 ( Pt 10):3219-31. [Abstract] [Full Text]
  • *Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, Rudensky AY, Ossendorp F, Melief CJ, Stoorvogel W, Geuze HJ: Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J Cell Biol. 2001 Oct 1;155(1):53-63. [Abstract] [Full Text]
  • *Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY: Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo. J Immunol. 2005 Feb 1;174(3):1205-12. [Abstract] [Full Text]
  • *Kuwano Y, Prazma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, Okochi H, Tamaki K, Fujimoto M, Tedder TF: CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells. Int Immunol. 2007 Aug;19(8):977-92. [Abstract] [Full Text]
  • *Zang W, Kalache S, Lin M, Schroppel B, Murphy B: MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J Am Soc Nephrol. 2005 Dec;16(12):3661-8. [Abstract] [Full Text]
  • *Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, F÷rster I, Reith W: Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice. Immunity. 1998 Feb;8(2):143-55. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    M5-114


    Fig. 1. Surface staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO┤s term and conditions which are available at www.exbio.cz.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy