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Mouse Monoclonal to FoxP3

3G3 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-601-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-601-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  A4-601-C025 Alexa Fluor® 488 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  A4-601-C100 Alexa Fluor® 488 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-601-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-601-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-601-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-601-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  A6-601-C025 Alexa Fluor® 647 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  A6-601-C100 Alexa Fluor® 647 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

FoxP3 (Forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.

Specificity

The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor, which is the master regulator in the development and function of regulatory T cells.

Regulatory Status

Immunogen

Full-length His-tagged recombinant murine FoxP3

Species Reactivity:

  • Human
  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
    Application note:
    intracellular staining
  • Western Blotting
    Recommended dilution:2 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr Opin Immunol. 2009 Dec;21(6):612-8. [Abstract]
  • *Barnes MJ, Powrie F: Regulatory T cells reinforce intestinal homeostasis. Immunity. 2009 Sep 18;31(3):401-11. [Abstract]
  • *Kuhn A, Beissert S, Krammer PH: CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch Dermatol Res. 2009 Jan;301(1):71-81. [Abstract]
  • *Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem Immunol Allergy. 2008;94:150-7. [Abstract]
  • *Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009 Oct 29;114(18):3727-35. [Abstract]
  • Product Specific References

  • *Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6659-64. [Abstract] [Full Text]
  • *Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009 Dec;75(12):1040-50. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    FoxP3
    Fig. 1.


    Fig. 1. Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3).


    3G3 PE


    Fig. 2. Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3) PE.



    1A-601


    Fig. 3. Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3) APC.



    A4-601


    Fig. 4. Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3) Alexa Fluor® 488.

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