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Mouse Monoclonal to CD140b / PDGF-RB

18A2 (IgG1)

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  11-590-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1P-590-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD140b / PDGF-RB (platelet-derived growth factor receptor beta) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140b forms homodimers, or heterodimerizes with CD140a / PDGF-RA. Whereas CD140a can have both pro-proliferative or anti-proliferative effects, the CD140b induces in various cell types their proliferation and migration. CD140b has also developmental roles in the cardiovascular system and is preferentially expressed on some tumours such as medulloblastoma.

Specificity

The monoclonal antibody 18A2 recognizes CD140b / PDGF-RB, the 180-190 kDa beta chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative roles.

Regulatory Status

Immunogen

CD140b-transfected NIH 3T3 cells

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1-5 μg/ml
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Andrae J, Gallini R, Betsholtz C: Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 2008 May 15;22(10):1276-312. [Abstract] [Full Text]
  • *French WJ, Creemers EE, Tallquist MD: Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol. 2008 Sep;28(18):5646-57. [Abstract] [Full Text]
  • *Schmahl J, Rizzolo K, Soriano P: The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev. 2008 Dec 1;22(23):3255-67. [Abstract] [Full Text]
  • *Faraone D, Aguzzi MS, Toietta G, Facchiano AM, Facchiano F, Magenta A, Martelli F, Truffa S, Cesareo E, Ribatti D, Capogrossi MC, Facchiano A: Platelet-derived growth factor-receptor alpha strongly inhibits melanoma growth in vitro and in vivo. Neoplasia. 2009 Aug;11(8):732-42. [Abstract] [Full Text]
  • Product Specific References

  • *Fekete N, Rojewski MT, Fürst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H: GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC. PLoS One. 2012;7(8):e43255. doi: 10.1371/journal.pone.0043255 [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    18a2 purified FC


    Fig. 1. Surface staining of CD140b / PDGF-RB in human CD140b / PDGF-RB stable transfectants with anti-CD140b (18A2) purified GAM-APC.


    18A2 APC


    Fig. 2. Surface staining of CD140b / PDGF-RB in human CD140b / PDGF-RB stable transfectants with anti-CD140b (18A2) APC.


    18A2 PE


    Fig. 3. Flow cytometry analysis (surface staining) of CD140b / PDGF-RB in human CD140b / PDGF-RB stable transfectants with anti-CD140b (18A2) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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