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Mouse Monoclonal to CD140a / PDGF-RA

16A1 (IgG1)

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Background

CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b / PDGF-RB. Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions.

Specificity

The mouse monoclonal antibody 16A1 recognizes CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.
HLDA VI.; WS Code E022

Regulatory Status

Immunogen

CD140a-transfected NIH 3T3 cells

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:2 μg/ml
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Andrae J, Gallini R, Betsholtz C: Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 2008 May 15;22(10):1276-312. [Abstract] [Full Text]
  • *French WJ, Creemers EE, Tallquist MD: Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol. 2008 Sep;28(18):5646-57. [Abstract] [Full Text]
  • *Schmahl J, Rizzolo K, Soriano P: The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev. 2008 Dec 1;22(23):3255-67. [Abstract] [Full Text]
  • *Faraone D, Aguzzi MS, Toietta G, Facchiano AM, Facchiano F, Magenta A, Martelli F, Truffa S, Cesareo E, Ribatti D, Capogrossi MC, Facchiano A: Platelet-derived growth factor-receptor alpha strongly inhibits melanoma growth in vitro and in vivo. Neoplasia. 2009 Aug;11(8):732-42. [Abstract] [Full Text]
  • Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    16A1 APC


    Fig. 1. Surface staining of CD140a in CD140a-transfected 3T3 cells using anti-CD140a (16A1) APC.


    16A1 PE


    Fig. 2. Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) PE.



    16A1 FITC


    Fig. 3. Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) FITC.


    16A1 biotin


    Fig. 4. Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) biotin / streptavidin-APC.


    16A1 pur


    Fig. 5. Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) purified / GAM-APC.

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