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Mouse Monoclonal to CD203c

NP4D6 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-585-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-585-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-585-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-585-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-585-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-585-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-585-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-585-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.

Specificity

The mouse monoclonal antibody NP4D6 reacts with CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.
HLDA VIII

Regulatory Status

Immunogen

HEK-293 cells transfected with human CD203c

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1-5 μg/ml
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Hauswirth AW, Escribano L, Prados A, Nuñez R, Mirkina I, Kneidinger M, Florian S, Sonneck K, Vales A, Schernthaner GH, Sanchez-Muñoz L, Sperr WR, Bühring HJ, Orfao A, Valent P: CD203c is overexpressed on neoplastic mast cells in systemic mastocytosis and is upregulated upon IgE receptor cross-linking. Int J Immunopathol Pharmacol. 2008 Oct-Dec;21(4):797-806. [Abstract]
  • *Tokuda R, Nagao M, Hiraguchi Y, Hosoki K, Matsuda T, Kouno K, Morita E, Fujisawa T: Antigen-induced expression of CD203c on basophils predicts IgE-mediated wheat allergy. Allergol Int. 2009 Jun;58(2):193-9. [Abstract]
  • *Wanich N, Nowak-Wegrzyn A, Sampson HA, Shreffler WG: Allergen-specific basophil suppression associated with clinical tolerance in patients with milk allergy. J Allergy Clin Immunol. 2009 Apr;123(4):789-94.e20. [Abstract]
  • *Chirumbolo S, Brizzi M, Ortolani R, Vella A, Bellavite P: Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study. Inflamm Res. 2009 Nov;58(11):755-64. [Abstract] [Full Text]
  • Product Specific References

  • *Bühring HJ, Simmons PJ, Pudney M, Müller R, Jarrossay D, van Agthoven A, Willheim M, Brugger W, Valent P, Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood. 1999 Oct 1;94(7):2343-56. [Abstract] [Full Text]
  • *Bühring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K: The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3. 1. Blood. 2001 May 15;97(10):3303-5. [Abstract] [Full Text]
  • *Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Bühring HJ: Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals. Int Arch Allergy Immunol. 2001 Dec;126(4):335-42. [Abstract]
  • *Heneberg P, Riegerová K, Kuèera P: Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro. PLoS One. 2015 Nov 12;10(11):e0142953. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    CD203c PE
    Fig. 1.


    Fig. 1. Surface staining of human basophils in allergen-stimulated whole blood (CD203cpos/ SSClow) by anti-CD203c antibody (NP4D6).


    NP4D6 ICC
    Fig. 2.


    Fig. 2. Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, green) and PE-conjugated anti-CD203c (clone NP4D6, red); merged signal yellow. DNA visualized by DAPI (blue).


    NP4D6 APC


    Fig. 3. Surface staining of human basophils in IgE-activated whole blood by anti-CD203c antibody (NP4D6) APC.


    1F-585


    Fig. 4. Surface staining of human peripheral blood by anti-CD203c antibody (NP4D6) FITC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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