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Rat Monoclonal to CD11b (mouse) / Mac-1 alpha

M1/70 (IgG2b)

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New 11-595-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  12-595-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-595-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-595-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-595-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-595-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-595-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

Specificity

The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.

Regulatory Status

Immunogen

B10 mouse spleen cells enriched for T cells

Species Reactivity:

  • Human
  • Non-Human Primates
  • Mouse
  • Rabbit

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1 μg/ml
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Functional Application
    In vitro blocking of CD11b
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Springer T, Galfrè G, Secher DS, Milstein C: Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug;8(8):539-51. [Abstract]
  • *Ault KA, Springer TA: Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981 Jan;126(1):359-64. [Abstract]
  • *Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983 Aug 1;158(2):586-602. [Abstract] [Full Text]
  • *Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ: Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 1995 Mar;43(3):313-20. [Abstract] [Full Text]
  • *Dembic Z, Schenck K, Bogen B: Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2697-702. [Abstract] [Full Text]
  • *Welt FG, Edelman ER, Simon DI, Rogers C: Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol. 2000 Dec;20(12):2553-8. [Abstract] [Full Text]
  • *Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC: Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 2002 Mar 15;168(6):3088-98. [Abstract] [Full Text]
  • *Brickson S, Ji LL, Schell K, Olabisi R, St Pierre Schneider B, Best TM: M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury. J Appl Physiol. 2003 Sep;95(3):969-76. [Abstract] [Full Text]
  • *Leon F, Contractor N, Fuss I, Marth T, Lahey E, Iwaki S, la Sala A, Hoffmann V, Strober W, Kelsall BL: Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis. J Immunol. 2006 Nov 15;177(10):6974-82. [Abstract] [Full Text]
  • *Takagi H, Numazaki M, Kajiwara T, Abe Y, Ishii M, Kato C, Kojima N: Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages. Glycobiology. 2009 Mar;19(3):258-66. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    M1-70 PE


    Fig. 1. Surface staining of CD11b in murine peritoneal fluid with anti-CD11b (M1/70) PE.


    M1-70 APC


    Fig. 2. Surface staining of CD11b in murine peritoneal fluid with anti-CD11b (M1/70) APC.

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