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Rat Monoclonal to CD19 (mouse)

1D3 (IgG2a)

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Background

CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

Specificity

The rat monoclonal antibody 1D3 detects mouse CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.

Regulatory Status

Immunogen

Mouse CD19-transfected cell line

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Functional Application
    This antibody can induce down-regulation of CD19, affecting the proportions of B cell subpopulations.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Krop I, Shaffer AL, Fearon DT, Schlissel MS: The signaling activity of murine CD19 is regulated during cell development. J Immunol. 1996 Jul 1;157(1):48-56. [Abstract]
  • *Cherukuri A, Cheng PC, Pierce SK: The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens. J Immunol. 2001 Jul 1;167(1):163-72. [Abstract] [Full Text]
  • *Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT: Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan;26(1):238-42. [Abstract]
  • *Inabe K, Kurosaki T: Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99(2):584-9. [Abstract] [Full Text]
  • *Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S: The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment. J Immunol. 2003 Oct 15;171(8):4062-72. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    1D3 PE FC


    Fig. 1. Surface staining of CD19 in murine splenocytes with anti-CD19 (1D3) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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