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Mouse Monoclonal to Actin

HHF35 (IgG1)

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Background

Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.

Specificity

The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.

Regulatory Status

Immunogen

SDS extracted protein fraction of human myocardium

Species Reactivity:

  • Human
  • Non-Human Primates
  • Mouse
  • Rat
  • Canine (Dog)
  • Feline (Cat)
  • Rabbit
  • Chicken

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution:1 μg/ml
    Positive control:murine femoral muscle, murine heart
    Negative control:HUVEC line
    Application note:reducing conditions
  • Immunohistochemistry (paraffin sections)
    Application note:Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Tsukada T, Tippens D, Gordon D, Ross R, Gown AM: HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am J Pathol. 1987 Jan;126(1):51-60. [Abstract] [Full Text]
  • *Tsukada T, McNutt MA, Ross R, Gown AM: HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues. Am J Pathol. 1987 May;127(2):389-402. [Abstract] [Full Text]
  • *Schmidt RA, Cone R, Haas JE, Gown AM: Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins. Am J Pathol. 1988 Apr;131(1):19-28. [Abstract] [Full Text]
  • *Miettinen M: Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors. Am J Pathol. 1988 Jan;130(1):205-15. [Abstract] [Full Text]
  • *de Sousa SO, Schwarzschild M, de Ara˙jo NS, de Ara˙jo VC: Basal cell adenocarcinoma of the palate with squamous metaplasia. J Clin Pathol. 2000 Feb;53(2):153-6. [Abstract] [Full Text]
  • *Handharyani E, Ochiai K, Iwata N, Umemura T: Immunohistochemical and ultrastructural study of ito cells (fat-storing cells) in response to extrahepatic bile duct ligation in broiler chickens. J Vet Med Sci. 2001 May;63(5):547-52. [Abstract] [Full Text]
  • *Ijzer J, Roskams T, Molenbeek RF, Ultee T, Penning LC, Rothuizen J, van den Ingh TS: Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver. Comp Hepatol. 2006 Nov 16;5:7. [Abstract] [Full Text]
  • *Matsuyama A, Hisaoka M, Hashimoto H: Angioleiomyoma: a clinicopathologic and immunohistochemical reappraisal with special reference to the correlation with myopericytoma. Hum Pathol. 2007 Apr;38(4):645-51. [Abstract]
  • *Matsuo S, Susumu S, Tsutsumi R, Azuma T, Obata S, Hayashi T: Glomus tumor of the omentum: a case report. J Surg Oncol. 2007 Dec 1;96(7):633-6. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    HHF35
    Fig. 1.


    Fig. 1. Western blotting analysis of muscle-specific actin using the monoclonal antibody HHF35.
    Lane 1: murine femoral muscle
    Lane 2: murine heart
    Lane 3: HeLa
    Lane 4: HUVEC

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