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Mouse Monoclonal to Lamin C

EM-11 (IgG2a)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-563-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-563-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Lamin C is intermediate filament protein localized to the inner nuclear membrane. It is expressed predominantly in terminally differentiated cells and defines the shape and stability of nuclei in mammalian cells. Besides their structural roles, lamin proteins also regulate fundamental aspects of nuclear function and they cross-talk with cell signaling cascades and cell metabolism. Mutations of LMNA gene, encoding lamin A and C proteins, are often associated with pathogenesis of respective cell types, such as of heart myocytes.

Specificity

The mouse monoclonal antibody EM-11 recognizes lamin C, intermediate filament protein of nuclear lamina.

Regulatory Status

Species Reactivity:

  • Human
  • Mouse
  • Other not determined

Negative Species:

Applications:

  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
    Application note:Paraformaldehyde fixation possible.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Mounkes LC, Burke B, Stewart CL: The A-type lamins: nuclear structural proteins as a focus for muscular dystrophy and cardiovascular diseases. Trends Cardiovasc Med. 2001 Oct;11(7):280-5. [Abstract]
  • *Saga A, Karibe A, Otomo J, Iwabuchi K, Takahashi T, Kanno H, Kikuchi J, Keitoku M, Shinozaki T, Shimokawa H: Lamin A/C gene mutations in familial cardiomyopathy with advanced atrioventricular block and arrhythmia. Tohoku J Exp Med. 2009 Aug;218(4):309-16. [Abstract] [Full Text]
  • *Chen S, Martin C, Maya-Mendoza A, Tang CW, Lovrić J, Sims PF, Jackson DA: Reduced Expression of Lamin A/C Results in Modified Cell Signaling and Metabolism Coupled with Changes in Expression of Structural Proteins. J Proteome Res. 2009 Oct 15. [Epub ahead of print] [Abstract]
  • *Cortés R, Roselló-Lletí E, Rivera M, Martínez-Dolz L, Azorín I, Salvador A, Portolés M: The role of the nuclear lamins in the pathogenesis of heart failure in patients undergoing cardiac transplantation. Transplant Proc. 2009 Jul-Aug;41(6):2227-30. [Abstract]
  • Product Specific References

  • *Shimi T, Kittisopikul M, Tran J, Goldman AE, Adam SA, Zheng Y, Jaqaman K, Goldman RD: Structural organization of nuclear lamins A, C, B1, and B2 revealed by superresolution microscopy. Mol Biol Cell. 2015 Nov 5;26(22):4075-86. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    lamin 1
    Fig. 1.

    Fig. 1. Immunofluorescence staining of lamin C (red) in HeLa cells by monoclonal antibody EM-11. Actin decorated by phalloidin-Alexa Fluor® 488 (green), DNA stained by DAPI (blue).


    lamin 2
    Fig. 2.

    Fig. 2. Immunofluorescence staining of lamin C (green) in mouse EL-6 fibroblasts by monoclonal antibody EM-11. DNA stained by DAPI (blue).

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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