categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD106 / VCAM-1

STA (IgG1)

Product No.	Form	Quantity	Price
11-606-C025	purified	0.025 mg	choose region
11-606-C100	purified	0.1 mg	choose region
1B-606-C025	biotin	0.025 mg	choose region
1B-606-C100	biotin	0.1 mg	choose region
1P-606-T025	PE	25 tests	choose region
1P-606-T100	PE	100 tests	choose region
1A-606-T025	APC	25 tests	choose region
1A-606-T100	APC	100 tests	choose region

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Background

CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesion molecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule taking part in their positive selection and survival, as well as in adhesion, transendothelial migration and activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts. Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation, tumour cells can use overexpressed VCAM-1 to escape T cell immunity. Soluble form of VCAM-1 (sVCAM-1) is an inflammatory marker and can be used also in prognosis of subsequent cariovascular events following acute coronary syndromes.

Entrez Gene: 1p32-p31

Specificity

The mouse monoclonal antibody STA recognizes CD106 antigen (VCAM-1), a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a costimulation molecule.
HLDA V; WS Code A013

Immunogen

Human DS6 T cell line

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution:4-6 μg/ml Positive control:TNF-alpha activated HUVEC cells Immunoprecipitation Immunohistochemistry (frozen sections) Application note:acetone fixation ELISA Application note: capture mAb for soluble CD106 Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references van Wetering S, van den Berk N, van Buul JD, Mul FP, Lommerse I, Mous R, ten Klooster JP, Zwaginga JJ, Hordijk PL: VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration. Am J Physiol Cell Physiol. 2003 Aug;285(2):C343-52. [Abstract] [Full Text] Wu TC: The role of vascular cell adhesion molecule-1 in tumor immune evasion. Cancer Res. 2007 Jul 1;67(13):6003-6. [Abstract] Postadzhiyan AS, Tzontcheva AV, Kehayov I, Finkov B: Circulating soluble adhesion molecules ICAM-1 and VCAM-1 and their association with clinical outcome, troponin T and C-reactive protein in patients with acute coronary syndromes. Clin Biochem. 2007 Sep 19 [Abstract] Paessens LC, Singh SK, Fernandes RJ, van Kooyk Y: Vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) provide co-stimulation in positive selection along with survival of selected thymocytes. Mol Immunol. 2008 Jan;45(1):42-8. [Abstract] Product Specific References Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Leca G, Mansur SE, Bensussan A: Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform. J Immunol. 1995 Feb 1;154(3):1069-77. [Abstract] *Yen YT, Liao F, Hsiao CH, Kao CL, Chen YC, Wu-Hsieh BA: Modeling the early events of severe acute respiratory syndrome coronavirus infection in vitro. J Virol. 2006 Mar;80(6):2684-93. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:4-6 μg/ml
Positive control:TNF-alpha activated HUVEC cells
Immunoprecipitation
Immunohistochemistry (frozen sections)
Application note:acetone fixation
ELISA
Application note: capture mAb for soluble CD106

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*van Wetering S, van den Berk N, van Buul JD, Mul FP, Lommerse I, Mous R, ten Klooster JP, Zwaginga JJ, Hordijk PL: VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration. Am J Physiol Cell Physiol. 2003 Aug;285(2):C343-52. [Abstract] [Full Text]

*Wu TC: The role of vascular cell adhesion molecule-1 in tumor immune evasion. Cancer Res. 2007 Jul 1;67(13):6003-6. [Abstract]

*Postadzhiyan AS, Tzontcheva AV, Kehayov I, Finkov B: Circulating soluble adhesion molecules ICAM-1 and VCAM-1 and their association with clinical outcome, troponin T and C-reactive protein in patients with acute coronary syndromes. Clin Biochem. 2007 Sep 19 [Abstract]

*Paessens LC, Singh SK, Fernandes RJ, van Kooyk Y: Vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) provide co-stimulation in positive selection along with survival of selected thymocytes. Mol Immunol. 2008 Jan;45(1):42-8. [Abstract]

Product Specific References

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

*Leca G, Mansur SE, Bensussan A: Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform. J Immunol. 1995 Feb 1;154(3):1069-77. [Abstract]

*Yen YT, Liao F, Hsiao CH, Kao CL, Chen YC, Wu-Hsieh BA: Modeling the early events of severe acute respiratory syndrome coronavirus infection in vitro. J Virol. 2006 Mar;80(6):2684-93. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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