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Mouse Monoclonal to CD106 / VCAM-1

STA (IgG1)

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Background

CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesion molecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule taking part in their positive selection and survival, as well as in adhesion, transendothelial migration and activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts. Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation, tumour cells can use overexpressed VCAM-1 to escape T cell immunity. Soluble form of VCAM-1 (sVCAM-1) is an inflammatory marker and can be used also in prognosis of subsequent cariovascular events following acute coronary syndromes.

Specificity

The mouse monoclonal antibody STA recognizes CD106 antigen (VCAM-1), a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a costimulation molecule.
HLDA V; WS Code A013

Regulatory Status

Immunogen

Human DS6 T cell line

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:4-6 μg/ml
    Positive control:TNF-alpha activated HUVEC cells
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
    Application note:acetone fixation
  • ELISA
    Application note: capture mAb for soluble CD106
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *van Wetering S, van den Berk N, van Buul JD, Mul FP, Lommerse I, Mous R, ten Klooster JP, Zwaginga JJ, Hordijk PL: VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration. Am J Physiol Cell Physiol. 2003 Aug;285(2):C343-52. [Abstract] [Full Text]
  • *Wu TC: The role of vascular cell adhesion molecule-1 in tumor immune evasion. Cancer Res. 2007 Jul 1;67(13):6003-6. [Abstract]
  • *Postadzhiyan AS, Tzontcheva AV, Kehayov I, Finkov B: Circulating soluble adhesion molecules ICAM-1 and VCAM-1 and their association with clinical outcome, troponin T and C-reactive protein in patients with acute coronary syndromes. Clin Biochem. 2007 Sep 19 [Abstract]
  • *Paessens LC, Singh SK, Fernandes RJ, van Kooyk Y: Vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) provide co-stimulation in positive selection along with survival of selected thymocytes. Mol Immunol. 2008 Jan;45(1):42-8. [Abstract]
  • Product Specific References

  • *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • *Leca G, Mansur SE, Bensussan A: Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform. J Immunol. 1995 Feb 1;154(3):1069-77. [Abstract]
  • *Yen YT, Liao F, Hsiao CH, Kao CL, Chen YC, Wu-Hsieh BA: Modeling the early events of severe acute respiratory syndrome coronavirus infection in vitro. J Virol. 2006 Mar;80(6):2684-93. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    STA PE


    Fig. 1. Surface staining of TNF alpha-stimulated HUVEC cells with anti-CD106 (STA) PE.


    STA APC


    Fig. 2. Surface staining of HDLM-2 cells with anti-CD106 (STA) APC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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