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Mouse Monoclonal to CD117 / c-Kit

104D2 (IgG1)

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Background

CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.

Specificity

The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.
HLDA VI; WS Code C-30

Regulatory Status

Immunogen

MOLM-1 megakaryocytic cells

Species Reactivity:

  • Human
  • Non-Human Primates
  • Bovine

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1 μg/ml
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997 Jul 1;90(1):111-25. [Abstract] [Full Text]
  • *Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer. Blood. 1998 Feb 1;91(3):898-906. [Abstract] [Full Text]
  • *Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c-Kit. Blood. 1999 Sep 15;94(6):1979-86. [Abstract] [Full Text]
  • *Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000 Apr;49(2):97-110. [Abstract] [Full Text]
  • *Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117). Exp Hematol. 2000 Jun;28(6):660-71. [Abstract]
  • *Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk Res. 2006 Oct;30(10):1241-8. [Abstract]
  • *Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007 Jul 1;110(1):151-60. [Abstract] [Full Text]
  • *Stevenson KS, Mc Glynn L, Hodge M, Mc Linden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem Cells Dev. 2009 Mar 27. [Abstract]
  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *And other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    104D2 pur


    Fig. 1. Surface staining of human peripheral blood cells with anti-CD117 (104D2) purified.


    104D2 APC-Cy7


    Fig. 2. Surface staining of human peripheral blood cells with anti-CD117 (104D2) APC-CyTM7.


    104D2 PE-Cy7


    Fig. 3. Surface staining of human peripheral blood cells with anti-CD117 (104D2) PE-CyTM7.


    104D2 PC55


    Fig. 4. Surface staining of human peripheral blood cells with anti-CD117 (104D2) PerCP-CyTM5.5.


    104D2 PB


    Fig. 5. Surface staining of human peripheral blood cells with anti-CD117 (104D2) Pacific BlueTM.

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