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Hamster Monoclonal to CD28 (mouse)

37.51 (IgG)

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CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells.


The Syrian hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells.

Regulatory Status


Mouse T cell lymphoma EL-4 cells

Species Reactivity:

  • Mouse

Negative Species:


  • Flow Cytometry
    Recommended dilution: 6 μg / ml
  • Immunoprecipitation
    Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
    Recommended dilution: 1-4 μg / ml
  • Functional Application
    In vitro and in vivo T cell costimulation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Harding FA, McArthur JG, Gross JA, Raulet DH, Allison JP: CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature. 1992 Apr 16;356(6370):607-9. [Abstract]
  • *Gross JA, Callas E, Allison JP: Identification and distribution of the costimulatory receptor CD28 in the mouse. J Immunol. 1992 Jul 15;149(2):380-8. [Abstract]
  • *Albert MH, Yu XZ, Martin PJ, Anasetti C: Prevention of lethal acute GVHD with an agonistic CD28 antibody and rapamycin. Blood. 2005 Feb 1;105(3):1355-61. [Abstract] [Full Text]
  • *Zhang N, He YW: An essential role for c-FLIP in the efficient development of mature T lymphocytes. J Exp Med. 2005 Aug 1;202(3):395-404. [Abstract] [Full Text]
  • *Zhang N, He YW: The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes. J Immunol. 2005 Jun 1;174(11):6967-73. [Abstract] [Full Text]
  • *Perchonock CE, Fernando MC, Quinn WJ 3rd, Nguyen CT, Sun J, Shapiro MJ, Shapiro VS: Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006 Aug;26(16):6005-15. [Abstract] [Full Text]
  • *Pua HH, Dzhagalov I, Chuck M, Mizushima N, He YW: A critical role for the autophagy gene Atg5 in T cell survival and proliferation. J Exp Med. 2007 Jan 22;204(1):25-31. [Abstract] [Full Text]
  • *Perchonock CE, Pajerowski AG, Nguyen C, Shapiro MJ, Shapiro VS: The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes. J Immunol. 2007 Aug 1;179(3):1768-75. [Abstract] [Full Text]
  • *Rosenberger CM, Clark AE, Treuting PM, Johnson CD, Aderem A: ATF3 regulates MCMV infection in mice by modulating IFN-gamma expression in natural killer cells. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2544-9. [Abstract] [Full Text]
  • *The long isoform of cellular FLIP is essential for T lymphocyte proliferation through an NF-kappaB-independent pathway. J Immunol. 2008 Apr 15;180(8):5506-11. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    Fig. 1. Surface staining of murine peritoneal fluid cells using anti-CD28 (clone 37.51) purified, GAArH-FITC.

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